Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed
Additional data
Additional Notes
Asynchronous cells were paraformaldehyde-fixed, permeablized, immunofluorescently labelled and counterstained with DAPI. This antibody did not produce the expected labeling pattern. MeOH-fixed samples were also evaluated and did not produce the expected labeling pattern.
Abcam response
Thank you for your valuable feedback. ab96861 has not been previously tested in ICC/IF. Your data suggests that ab96861 may not be suitable to detect Cullin 7 in human HeLa cells under these experimental condition. We hop this information is useful to our customers.
Dr. Kirk Mcmanus
Verified customer
Submitted Jan 02 2018