Key features and details
- Rabbit polyclonal to CWC15
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CWC15 antibody
DescriptionRabbit polyclonal to CWC15
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- WB: Human cell line RT4, Human cell line U-251 MG sp, Human plasma (IgG/HSA depleted), Human liver tissue and Human tonsil tissue lysates. IHC-P: Human thyroid gland. ICC/IF: U-2 OS cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab188345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 27 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceComponent of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Belongs to the CWC15 family.
- CWC15 antibody
- AD-002 antibody
- C11orf5 antibody
All lanes : Anti-CWC15 antibody (ab188345) at 1/100 dilution
Lane 2 : RT4 cell lysate
Lane 3 : U251 MG sp cell lysate
Lane 4 : Human plasma (IgG/HSA depleted)
Lane 5 : Human liver tissue lysate
Lane 6 : Human tonsil tissue lysate
Predicted band size: 27 kDa
U-2 OS cells stained for CWC15 (green) using ab188345 at 2 µg/ml in ICC/IF.
Paraffin embedded human thyroid gland tissue stained for CWC15 using ab188345 at 1/1000 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
ab188345 has not yet been referenced specifically in any publications.