Product nameAnti-CWF19L1 antibody
See all CWF19L1 primary antibodies
DescriptionRabbit polyclonal to CWF19L1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Human smooth muscle tissue; RT4 cell lysate; U 251 MG cell lysate; Human tonsil tissue lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab150842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/250. Predicted molecular weight: 61 kDa.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
RelevanceCWF19L1 belongs to the CWF19 family.
- CWF19 like protein 1 antibody
- CWF19-like 1, cell cycle control (S. pombe) antibody
- CWF19L1 antibody
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli and golgi apparatus. Recommended concentration of ab150842 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
All lanes : Anti-CWF19L1 antibody (ab150842) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 61 kDa
Immunohistochemical analysis of paraffin-embedded Human smooth muscle tissue labeling CWF19L1 with ab150842 at 1/500 dilution.
ab150842 has not yet been referenced specifically in any publications.