Overview

  • Product name

    Anti-Cx30 antibody [16H9L8]
    See all Cx30 primary antibodies
  • Description

    Rabbit monoclonal [16H9L8] to Cx30
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Cx30 aa 241-261.
    Sequence:

    QNEMNELISDSGQNAITSFPS


    Database link: P70689

  • Positive control

    • WB: Adult mouse thalamus lysates. IF: Log phase Caco-2 cells. IHC: Adult mouse thalamus tissue .
  • General notes

    Protein previously known as GJB6.

Properties

Applications

Our Abpromise guarantee covers the use of ab200866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 30 kDa.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • Involvement in disease

    Defects in GJB6 are the cause of ectodermal dysplasia type 2 (ED2) [MIM:129500]; also known as Clouston syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ED2 is an autosomal dominant condition characterized by atrichosis, nail hypoplasia and deformities, hyperpigmentation of the skin, normal teeth, normal sweat and sebaceous gland function. Palmoplantar hyperkeratosis is a frequent features. Hearing impairment has been detected in few cases of ED2.
    Defects in GJB6 are the cause of deafness autosomal recessive type 1B (DFNB1B) [MIM:612645]. DFNB1B is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
    Defects in GJB6 are the cause of deafness autosomal dominant type 3B (DFNA3B) [MIM:612643].
  • Sequence similarities

    Belongs to the connexin family. Beta-type (group I) subfamily.
  • Cellular localization

    Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • Database links

  • Alternative names

    • Connexin 30 antibody
    • Connexin-30 antibody
    • Cx30 antibody
    • CXB6_HUMAN antibody
    • DFNA3 antibody
    • DFNA3B antibody
    • DFNB1B antibody
    • ECTD2 antibody
    • ectodermal dysplasia 2, hidrotic (Clouston syndrome) antibody
    • ED2 antibody
    • EDH antibody
    • Gap junction beta 6 protein antibody
    • Gap junction beta-6 protein antibody
    • gap junction protein, beta 6 (connexin 30) antibody
    • gap junction protein, beta 6 antibody
    • Gap junction protein, beta 6, 30kDa antibody
    • GJB6 antibody
    • HED antibody
    • HED2 antibody
    • NSRD1 antibody
    see all

Images

  • Immunohistochemical analysis of adult mouse thalamus tissue labeling Cx30 with ab200866 at 0.5 µg/ml.

  • Anti-Cx30 antibody [16H9L8] (ab200866) at 1 µg/ml + Adult mouse thalamus lysate

    Predicted band size: 30 kDa

  • Immunofluorescence analysis of 4% paraformaldehyde-fixed log phase Caco-2 cells labeling Cx30 with ab200866 at 1/500 dilution. Alexa Fluor® 488 Goat Anti-Rabbit IgG Secondary Antibody at a dilution of 1/400 was used as the secondary antibody (panel a; green). Slide is counter stained with Dapi (panel b: blue). Panel c is a merged image showing cell junction localization and panel d is a no primary antibody control. The images were captured using a Nikon microscope at 20X magnification.

References

ab200866 has not yet been referenced specifically in any publications.

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