Key features and details
- Rabbit polyclonal to Cx36
- Suitable for: IHC-FoFr, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cx36 antibody
See all Cx36 primary antibodies
DescriptionRabbit polyclonal to Cx36
Tested applicationsSuitable for: IHC-FoFr, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
- Jurkat whole cell lysate (ab7899)
Protein previously known as Connexin 36/GJA9.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 2% Sucrose
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionOne gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Tissue specificityHighly expressed in neurons.
Sequence similaritiesBelongs to the connexin family. Delta-type subfamily.
Cellular localizationCell membrane. Cell junction > gap junction.
- Information by UniProt
- Connexin 36 / GJA9 antibody
- Connexin 36 antibody
- Connexin-36 antibody
Male mouse (P41) was anesthetized with isoflurane until they became non-responsive to tail pinch. Animal's thoracic cavity opened and it was transcardially perfused with 10 mL of ice cold PBS, then 10 mL of Ice cold 4 % PFA solution. Brain was removed and fixed overnight in 4% PFA. Next morning brain was rinsed 3X in PBS. Brain was immersed in agar and sliced at a thickness of 50uM. Brain slices were incubated overnight in a solution of PBST (0.2%) + 10% Normal GOAT serum + a monoclonal mouse anti-Parvalbumin antibody (1:2000)+ ab86408 (1ug/mL) and gently shaken overnight at 4°C. Samples were washed 6x in PBS (10 min each at RT) and exposed to secondary antibodies at 4°C: PBST (0.3%) + 10% normal goat serum + anti-mouse Alexa 488 (1:1000) + Anti-rabbit Alexa 555 (1:1000). Samples were washed 6x in PBST mounted. Images acquired with a confocal microscope at 63X.
Anti-Cx36 antibody (ab86408) at 1 µg/ml + Jurkat cell lysate at 10 µg
HRP-conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Gel concentration 12%
ab86408 has been referenced in 2 publications.
- Kovács-Öller T et al. Connexin36 Expression in the Mammalian Retina: A Multiple-Species Comparison. Front Cell Neurosci 11:65 (2017). PubMed: 28337128
- Franco-Pérez J et al. Sleep deprivation and sleep recovery modifies connexin36 and connexin43 protein levels in rat brain. Neuroreport 23:103-7 (2012). PubMed: 22158029