Product nameAnti-Cx37/GJA4 antibody
See all Cx37/GJA4 primary antibodies
DescriptionRabbit polyclonal to Cx37/GJA4
Tested applicationsSuitable for: IP, ELISA, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
- NIH/3T3 membrane lysate
Previously labelled as Connexin 37 / GJA4.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab58918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay, 1:312500.
|WB||Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionOne gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Tissue specificityExpressed in multiple organs and tissues, including heart, uterus, ovary, and blood vessel endothelium.
Sequence similaritiesBelongs to the connexin family. Alpha-type (group II) subfamily.
Cellular localizationCell membrane. Cell junction > gap junction.
- Information by UniProt
- Connexin 37 antibody
- Connexin-37 antibody
- Cx37 antibody
Cx37 / GJA4 was immunoprecipitated using 0.5mg NIH3T3 whole cell extract, 5µg of Rabbit polyclonal to Cx37 / GJA4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab58918.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 45kDa; Cx37 / GJA4
Anti-Cx37/GJA4 antibody (ab58918) at 10 µg/ml + NIH/3T3 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 37 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Yang Y et al. The Increased Expression of Connexin and VEGF in Mouse Ovarian Tissue Vitrification by Follicle Stimulating Hormone. Biomed Res Int 2015:397264 (2015). IHC (PFA fixed) ; Mouse . Read more (PubMed: 26539488) »
- Tsang H et al. Role of asymmetric methylarginine and connexin 43 in the regulation of pulmonary endothelial function. Pulm Circ 3:675-91 (2013). WB . Read more (PubMed: 24618552) »