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  1. Link

    cx37gja4-antibody-n-terminal-ab181701.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Gap Junctions
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Anti-Cx37/GJA4 antibody - N-terminal (ab181701)

  • Datasheet
  • SDS
Submit a review Submit a question References (5)

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  • Western blot - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)

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  • Datasheet
  • References (5)
  • Protocols

Overview

  • Product name

    Anti-Cx37/GJA4 antibody - N-terminal
    See all Cx37/GJA4 primary antibodies
  • Description

    Rabbit polyclonal to Cx37/GJA4 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human Cx37/GJA4 aa 3-17 (N terminal).
    Sequence:

    DWGFLEKLLDQVQEH


    Database link: P35212

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Rat heart tissue lysate. HELA, MCF7, SKOV, A431 and mouse NIH 3T3 cell lysates. Human intestinal cancer, rat cardiac muscle tissues. HeLa cells.
  • General notes

    Previously labelled as Connexin 37 / GJA4.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Gap Junctions
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell junction molecules

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • MCF7 whole cell lysate (ab3871)
    • NIH 3T3 whole cell lysate (ab7179)
    • A431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human Cx37/GJA4 protein (ab114495)
  • Related Products

    • Recombinant Human Cx37/GJA4 protein (ab114495)

Applications

Our Abpromise guarantee covers the use of ab181701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 37 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • Tissue specificity

    Expressed in multiple organs and tissues, including heart, uterus, ovary, and blood vessel endothelium.
  • Sequence similarities

    Belongs to the connexin family. Alpha-type (group II) subfamily.
  • Cellular localization

    Cell membrane. Cell junction > gap junction.
  • Target information above from: UniProt accession P35212 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 538913 Cow
    • Entrez Gene: 2701 Human
    • Entrez Gene: 14612 Mouse
    • Entrez Gene: 25655 Rat
    • Entrez Gene: 100335149 Xenopus laevis
    • Entrez Gene: 780366 Xenopus tropicalis
    • Omim: 121012 Human
    • SwissProt: A4IFL1 Cow
    • SwissProt: P35212 Human
    • SwissProt: P28235 Mouse
    • SwissProt: Q03190 Rat
    • SwissProt: P51914 Xenopus laevis
    • SwissProt: Q0V990 Xenopus tropicalis
    • Unigene: 296310 Human
    • Unigene: 24615 Mouse
    • Unigene: 23406 Rat
    see all
  • Alternative names

    • Connexin 37 antibody
    • Connexin-37 antibody
    • Cx37 antibody
    • CXA4_HUMAN antibody
    • Gap junction alpha 4 protein antibody
    • Gap junction alpha-4 protein antibody
    • gap junction protein, alpha 4 37kDa (connexin 37) antibody
    • gap junction protein, alpha 4, 37kD (connexin 37) antibody
    • Gap junction protein, alpha 4, 37kDa antibody
    • Gja4 antibody
    • OTTHUMP00000004181 antibody
    • OTTHUMP00000004182 antibody
    see all

Images

  • Western blot - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)
    Western blot - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)
    All lanes : Anti-Cx37/GJA4 antibody - N-terminal (ab181701) at 0.5 µg/ml

    Lane 1 : rat heart tissue lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : mouse NIH 3T3 cell lysate
    Lane 5 : SKOV cell lysate
    Lane 6 : A431 cell lysate

    Predicted band size: 37 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)

    Immunohistochemical analysis of paraffin-embedded Human intestinal cancer tissue, labeling Cx37 / GJA4 using ab181701 at 1 μg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue, labeling Cx37 / GJA4 using ab181701 at 1 μg/ml.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)
    Immunocytochemistry/ Immunofluorescence - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)

    Immunofluorescence analysis of HeLa cells, labeling Cx37 / GJA4 using ab181701 at 1 μg/ml.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References

    This product has been referenced in:

    • Choi D  et al. Piezo1 incorporates mechanical force signals into the genetic program that governs lymphatic valve development and maintenance. JCI Insight 4:N/A (2019). Read more (PubMed: 30676326) »
    • Lei R  et al. Effects of Fullerenol Nanoparticles on Rat Oocyte Meiosis Resumption. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29494500) »
    See all 5 Publications for this product

    Publishing research using ab181701? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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