Anti-Cx37/GJA4 antibody - N-terminal (ab181701)
Key features and details
- Rabbit polyclonal to Cx37/GJA4 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cx37/GJA4 antibody - N-terminal
See all Cx37/GJA4 primary antibodies -
Description
Rabbit polyclonal to Cx37/GJA4 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Xenopus laevis, Xenopus tropicalis
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Immunogen
Synthetic peptide corresponding to Human Cx37/GJA4 aa 3-17 (N terminal).
Sequence:DWGFLEKLLDQVQEH
Database link: P35212 -
Positive control
- Rat heart tissue lysate. HELA, MCF7, SKOV, A431 and mouse NIH 3T3 cell lysates. Human intestinal cancer, rat cardiac muscle tissues. HeLa cells.
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General notes
Previously labelled as Connexin 37 / GJA4.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab181701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 37 kDa. | |
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 0.5 - 1 µg/ml. |
Target
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Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. -
Tissue specificity
Expressed in multiple organs and tissues, including heart, uterus, ovary, and blood vessel endothelium. -
Sequence similarities
Belongs to the connexin family. Alpha-type (group II) subfamily. -
Cellular localization
Cell membrane. Cell junction > gap junction. - Information by UniProt
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Database links
- Entrez Gene: 538913 Cow
- Entrez Gene: 2701 Human
- Entrez Gene: 14612 Mouse
- Entrez Gene: 25655 Rat
- Entrez Gene: 100335149 Xenopus laevis
- Entrez Gene: 780366 Xenopus tropicalis
- Omim: 121012 Human
- SwissProt: A4IFL1 Cow
see all -
Alternative names
- Connexin 37 antibody
- Connexin-37 antibody
- Cx37 antibody
see all
Images
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Western blot - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)All lanes : Anti-Cx37/GJA4 antibody - N-terminal (ab181701) at 0.5 µg/ml
Lane 1 : rat heart tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : mouse NIH 3T3 cell lysate
Lane 5 : SKOV cell lysate
Lane 6 : A431 cell lysate
Predicted band size: 37 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)Immunohistochemical analysis of paraffin-embedded Human intestinal cancer tissue, labeling Cx37 / GJA4 using ab181701 at 1 μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue, labeling Cx37 / GJA4 using ab181701 at 1 μg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)Immunofluorescence analysis of HeLa cells, labeling Cx37 / GJA4 using ab181701 at 1 μg/ml.
Protocols
References (7)
ab181701 has been referenced in 7 publications.
- Jamalzaei P et al. Effects of Alginate Concentration and Ovarian Cells on In Vitro Development of Mouse Preantral Follicles: A Factorial Study. Int J Fertil Steril 13:330-338 (2020). PubMed: 31710195
- Kosovic I et al. Spatio-temporal patterning of different connexins in developing and postnatal human kidneys and in nephrotic syndrome of the Finnish type (CNF). Sci Rep 10:8756 (2020). PubMed: 32471989
- Choi D et al. Piezo1 incorporates mechanical force signals into the genetic program that governs lymphatic valve development and maintenance. JCI Insight 4:N/A (2019). PubMed: 30676326
- Lei R et al. Effects of Fullerenol Nanoparticles on Rat Oocyte Meiosis Resumption. Int J Mol Sci 19:N/A (2018). PubMed: 29494500
- Donfack NJ et al. Stroma cell-derived factor 1 and connexins (37 and 43) are preserved after vitrification and in vitro culture of goat ovarian cortex. Theriogenology 116:83-88 (2018). PubMed: 29783047
- Jiang C et al. GGPP-Mediated Protein Geranylgeranylation in Oocyte Is Essential for the Establishment of Oocyte-Granulosa Cell Communication and Primary-Secondary Follicle Transition in Mouse Ovary. PLoS Genet 13:e1006535 (2017). PubMed: 28072828
- Fang JS et al. Shear-induced Notch-Cx37-p27 axis arrests endothelial cell cycle to enable arterial specification. Nat Commun 8:2149 (2017). PubMed: 29247167
