Anti-Cx37/GJA4 antibody - N-terminal (ab181701)
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Anti-Cx37/GJA4 antibody - N-terminal
See all Cx37/GJA4 primary antibodies -
Description
Rabbit polyclonal to Cx37/GJA4 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Xenopus laevis, Xenopus tropicalis
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Immunogen
Synthetic peptide corresponding to Human Cx37/GJA4 aa 3-17 (N terminal).
Sequence:DWGFLEKLLDQVQEH
Database link: P35212 -
Positive control
- Rat heart tissue lysate. HELA, MCF7, SKOV, A431 and mouse NIH 3T3 cell lysates. Human intestinal cancer, rat cardiac muscle tissues. HeLa cells.
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General notes
Previously labelled as Connexin 37 / GJA4.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab181701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 37 kDa. | |
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 0.5 - 1 µg/ml. |
Target
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Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. -
Tissue specificity
Expressed in multiple organs and tissues, including heart, uterus, ovary, and blood vessel endothelium. -
Sequence similarities
Belongs to the connexin family. Alpha-type (group II) subfamily. -
Cellular localization
Cell membrane. Cell junction > gap junction. - Information by UniProt
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Database links
- Entrez Gene: 538913 Cow
- Entrez Gene: 2701 Human
- Entrez Gene: 14612 Mouse
- Entrez Gene: 25655 Rat
- Entrez Gene: 100335149 Xenopus laevis
- Entrez Gene: 780366 Xenopus tropicalis
- Omim: 121012 Human
- SwissProt: A4IFL1 Cow
see all -
Alternative names
- Connexin 37 antibody
- Connexin-37 antibody
- Cx37 antibody
see all
Images
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Western blot - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)All lanes : Anti-Cx37/GJA4 antibody - N-terminal (ab181701) at 0.5 µg/ml
Lane 1 : rat heart tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : mouse NIH 3T3 cell lysate
Lane 5 : SKOV cell lysate
Lane 6 : A431 cell lysate
Predicted band size: 37 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)Immunohistochemical analysis of paraffin-embedded Human intestinal cancer tissue, labeling Cx37 / GJA4 using ab181701 at 1 μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue, labeling Cx37 / GJA4 using ab181701 at 1 μg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-Cx37/GJA4 antibody - N-terminal (ab181701)Immunofluorescence analysis of HeLa cells, labeling Cx37 / GJA4 using ab181701 at 1 μg/ml.
Protocols
References
This product has been referenced in:
- Choi D et al. Piezo1 incorporates mechanical force signals into the genetic program that governs lymphatic valve development and maintenance. JCI Insight 4:N/A (2019). Read more (PubMed: 30676326) »
- Lei R et al. Effects of Fullerenol Nanoparticles on Rat Oocyte Meiosis Resumption. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29494500) »
