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  1. Link

    cx3cl1-antibody-ab25088.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-CX3CL1 antibody (ab25088)

  • Datasheet
  • SDS
Reviews (6)Q&A (1)References (57)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CL1 antibody (ab25088)
  • Immunocytochemistry/ Immunofluorescence - Anti-CX3CL1 antibody (ab25088)

Key features and details

  • Rabbit polyclonal to CX3CL1
  • Suitable for: IHC-Fr, ICC/IF, IHC-FoFr, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CX3CL1 antibody
    See all CX3CL1 primary antibodies
  • Description

    Rabbit polyclonal to CX3CL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, IHC-FoFr, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: E. coli expressed chemotactic domain of human CX3CL1

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Chemokines
    • Delta Chemokines (CXC3)
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human CX3CL1 protein (ab9820)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab25088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (1)
Use at an assay dependent concentration.
ICC/IF (1)
Use a concentration of 1 µg/ml.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 22387113
WB (4)
Use at an assay dependent concentration.
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 1 µg/ml.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 22387113
WB
Use at an assay dependent concentration.
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.
  • Tissue specificity

    Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Belongs to the intercrine delta family.
  • Post-translational
    modifications

    A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P78423 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6376 Human
    • Entrez Gene: 20312 Mouse
    • Entrez Gene: 89808 Rat
    • Omim: 601880 Human
    • SwissProt: P78423 Human
    • SwissProt: O35188 Mouse
    • SwissProt: O55145 Rat
    • Unigene: 531668 Human
    • Unigene: 103711 Mouse
    • Unigene: 107266 Rat
    see all
  • Alternative names

    • A 152E5.2 antibody
    • AB030188 antibody
    • ABCD 3 antibody
    • ABCD3 antibody
    • AI848747 antibody
    • C-X3-C motif chemokine 1 antibody
    • C3Xkine antibody
    • Chemokine (C-X3-C motif) ligand 1 antibody
    • Chemokine C X3 C motif ligand 1 antibody
    • Chemokine CX3C Motif Ligand 1 antibody
    • CX3C membrane anchored chemokine antibody
    • CX3C membrane-anchored chemokine antibody
    • Cx3cl1 antibody
    • CXC 3 antibody
    • CXC3 antibody
    • CXC3C antibody
    • D8Bwg0439e antibody
    • FKN antibody
    • Fractalkine antibody
    • Neurotactin antibody
    • NTN antibody
    • NTT antibody
    • Processed fractalkine antibody
    • SCYD 1 antibody
    • SCYD1 antibody
    • Small inducible cytokine D1 antibody
    • Small inducible cytokine subfamily D (Cys X3 Cys) member 1 antibody
    • small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin) antibody
    • Small inducible cytokine subfamily D member 1 antibody
    • Small-inducible cytokine D1 antibody
    • X3CL1_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CL1 antibody (ab25088)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CL1 antibody (ab25088)
    ab25088 (4µg/ml) staining CX3CL1 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasmic region of the cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunocytochemistry/ Immunofluorescence - Anti-CX3CL1 antibody (ab25088)
    Immunocytochemistry/ Immunofluorescence - Anti-CX3CL1 antibody (ab25088)

    ICC/IF image of ab25088 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25088, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (57)

Publishing research using ab25088? Please let us know so that we can cite the reference in this datasheet.

ab25088 has been referenced in 57 publications.

  • Li H  et al. STAT3/miR-15a-5p/CX3CL1 Loop Regulates Proliferation and Migration of Vascular Endothelial Cells in Atherosclerosis. Int J Med Sci 18:964-974 (2021). PubMed: 33456354
  • Que ZJ  et al. Jinfukang inhibits lung cancer metastasis by upregulating CX3CL1 to recruit NK cells to kill CTCs. J Ethnopharmacol 275:114175 (2021). PubMed: 33933571
  • Kawamura N  et al. Impaired brain fractalkine-CX3CR1 signaling is implicated in cognitive dysfunction in diet-induced obese mice. BMJ Open Diabetes Res Care 9:N/A (2021). PubMed: 33568358
  • Wang K  et al. Vertebral-specific activation of the CX3CL1/ICAM-1 signaling network mediates non-small-cell lung cancer spinal metastasis by engaging tumor cell-vertebral bone marrow endothelial cell interactions. Theranostics 11:4770-4789 (2021). PubMed: 33754027
  • Fernández-Albarral JA  et al. Retinal Molecular Changes Are Associated with Neuroinflammation and Loss of RGCs in an Experimental Model of Glaucoma. Int J Mol Sci 22:N/A (2021). PubMed: 33669765
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-CX3CL1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 13 2020

Immunohistochemistry (Frozen sections) abreview for Anti-CX3CL1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 10 2020

Western blot abreview for Anti-CX3CL1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary astrocytes)
Loading amount
2 µg
Specification
Primary astrocytes
Gel Running Conditions
Reduced Denaturing (gel 10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 16 2013

Western blot abreview for Anti-CX3CL1 antibody

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (endothelial cell)
Loading amount
80000 cells
Specification
endothelial cell
Gel Running Conditions
Reduced Denaturing (gel 6%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 01 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-CX3CL1 antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (endothelial cell)
Specification
endothelial cell
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 16 2012

Western blot abreview for Anti-CX3CL1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Recombinant protein (recombinant protein)
Loading amount
0.025 µg
Specification
recombinant protein
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 16 2007

Question

Hi,
I was wondering if Anti-CX3CL1 (ab25088) antibody can be used on PFA-fixed
paraffin-embedded tissue sections on immunofluorescence application. On
the web-site it only says Immunohistochemistry for PFA-fixed paraffin
embedded sections.

Read More

Abcam community

Verified customer

Asked on Oct 02 2012

Answer

Thank you for contacting us. We consider IHC-P to be staining of tissue in paraffin embedded tissue sections. We use the term immunofluorescence or (or immunocytochemistry) to mean staining of cells.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Oct 02 2012

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