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    cx3cl1-antibody-ab25088.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-CX3CL1 antibody (ab25088)

  • Datasheet
  • SDS
Reviews (6)Q&A (1)References (37)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CL1 antibody (ab25088)
  • Immunocytochemistry/ Immunofluorescence - Anti-CX3CL1 antibody (ab25088)

Key features and details

  • Rabbit polyclonal to CX3CL1
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CX3CL1 antibody
    See all CX3CL1 primary antibodies
  • Description

    Rabbit polyclonal to CX3CL1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment: E. coli expressed chemotactic domain of human CX3CL1

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Chemokines
    • Delta Chemokines (CXC3)
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human CX3CL1 protein (ab9820)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab25088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
Application Abreviews Notes
ICC/IF (1)
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.
  • Tissue specificity

    Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Belongs to the intercrine delta family.
  • Post-translational
    modifications

    A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P78423 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6376 Human
    • Omim: 601880 Human
    • SwissProt: P78423 Human
    • Unigene: 531668 Human
    • Alternative names

      • A 152E5.2 antibody
      • AB030188 antibody
      • ABCD 3 antibody
      • ABCD3 antibody
      • AI848747 antibody
      • C-X3-C motif chemokine 1 antibody
      • C3Xkine antibody
      • Chemokine (C-X3-C motif) ligand 1 antibody
      • Chemokine C X3 C motif ligand 1 antibody
      • Chemokine CX3C Motif Ligand 1 antibody
      • CX3C membrane anchored chemokine antibody
      • CX3C membrane-anchored chemokine antibody
      • Cx3cl1 antibody
      • CXC 3 antibody
      • CXC3 antibody
      • CXC3C antibody
      • D8Bwg0439e antibody
      • FKN antibody
      • Fractalkine antibody
      • Neurotactin antibody
      • NTN antibody
      • NTT antibody
      • Processed fractalkine antibody
      • SCYD 1 antibody
      • SCYD1 antibody
      • Small inducible cytokine D1 antibody
      • Small inducible cytokine subfamily D (Cys X3 Cys) member 1 antibody
      • small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin) antibody
      • Small inducible cytokine subfamily D member 1 antibody
      • Small-inducible cytokine D1 antibody
      • X3CL1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CL1 antibody (ab25088)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CL1 antibody (ab25088)
      ab25088 (4µg/ml) staining CX3CL1 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasmic region of the cells.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
    • Immunocytochemistry/ Immunofluorescence - Anti-CX3CL1 antibody (ab25088)
      Immunocytochemistry/ Immunofluorescence - Anti-CX3CL1 antibody (ab25088)

      ICC/IF image of ab25088 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25088, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (37)

    Publishing research using ab25088? Please let us know so that we can cite the reference in this datasheet.

    ab25088 has been referenced in 37 publications.

    • Linker KE  et al. Microglial activation increases cocaine self-administration following adolescent nicotine exposure. Nat Commun 11:306 (2020). PubMed: 31949158
    • Hill SL  et al. Diminished levels of the chemokine fractalkine in post-mortem prefrontal cortex in schizophrenia but not bipolar disorder. World J Biol Psychiatry N/A:1-10 (2020). PubMed: 32295454
    • Chen G  et al. A novel CX3CR1 inhibitor AZD8797 facilitates early recovery of rat acute spinal cord injury by inhibiting inflammation and apoptosis. Int J Mol Med 45:1373-1384 (2020). PubMed: 32323731
    • Wang SK  et al. Soluble CX3CL1 gene therapy improves cone survival and function in mouse models of retinitis pigmentosa. Proc Natl Acad Sci U S A 116:10140-10149 (2019). PubMed: 31036641
    • Fu D  et al. FKN Facilitates HK-2 Cell EMT and Tubulointerstitial Lesions via the Wnt/ß-Catenin Pathway in a Murine Model of Lupus Nephritis. Front Immunol 10:784 (2019). PubMed: 31134047
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-CX3CL1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    30 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 13 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-CX3CL1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Heart)
    Permeabilization
    Yes - Tween-20
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 10 2020

    Western blot abreview for Anti-CX3CL1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Primary astrocytes)
    Loading amount
    2 µg
    Specification
    Primary astrocytes
    Gel Running Conditions
    Reduced Denaturing (gel 10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 16 2013

    Question

    Hi,
    I was wondering if Anti-CX3CL1 (ab25088) antibody can be used on PFA-fixed
    paraffin-embedded tissue sections on immunofluorescence application. On
    the web-site it only says Immunohistochemistry for PFA-fixed paraffin
    embedded sections.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 02 2012

    Answer

    Thank you for contacting us. We consider IHC-P to be staining of tissue in paraffin embedded tissue sections. We use the term immunofluorescence or (or immunocytochemistry) to mean staining of cells.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 02 2012

    Western blot abreview for Anti-CX3CL1 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (endothelial cell)
    Loading amount
    80000 cells
    Specification
    endothelial cell
    Gel Running Conditions
    Reduced Denaturing (gel 6%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 01 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CX3CL1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (endothelial cell)
    Specification
    endothelial cell
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 16 2012

    Western blot abreview for Anti-CX3CL1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Recombinant protein (recombinant protein)
    Loading amount
    0.025 µg
    Specification
    recombinant protein
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 16 2007

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