Anti-CX3CL1 antibody (ab25088)

Rabbit polyclonal CX3CL1 antibody. Validated in WB, IHC, ICC/IF and tested in Mouse, Rat, Human. Cited in 26 publication(s). Independently reviewed in 4 review(s).


  • Product name
  • Description
    Rabbit polyclonal to CX3CL1
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: E. coli expressed chemotactic domain of human CX3CL1

  • Positive control
    • WB: Mouse recombinant protein. IHC-P: Human lung tissue. ICC/IF: HeLa cells.



Our Abpromise guarantee covers the use of ab25088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22387113
IHC-Fr 1/100 - 1/500.
IHC-P Use a concentration of 4 µg/ml.
WB 1/1000 - 1/5000. Predicted molecular weight: 42 kDa.


  • Function
    The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.
  • Tissue specificity
    Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas.
  • Sequence similarities
    Belongs to the intercrine delta family.
  • Post-translational
    A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A 152E5.2 antibody
    • AB030188 antibody
    • ABCD 3 antibody
    • ABCD3 antibody
    • AI848747 antibody
    • C-X3-C motif chemokine 1 antibody
    • C3Xkine antibody
    • Chemokine (C-X3-C motif) ligand 1 antibody
    • Chemokine C X3 C motif ligand 1 antibody
    • Chemokine CX3C Motif Ligand 1 antibody
    • CX3C membrane anchored chemokine antibody
    • CX3C membrane-anchored chemokine antibody
    • Cx3cl1 antibody
    • CXC 3 antibody
    • CXC3 antibody
    • CXC3C antibody
    • D8Bwg0439e antibody
    • FKN antibody
    • Fractalkine antibody
    • Neurotactin antibody
    • NTN antibody
    • NTT antibody
    • Processed fractalkine antibody
    • SCYD 1 antibody
    • SCYD1 antibody
    • Small inducible cytokine D1 antibody
    • Small inducible cytokine subfamily D (Cys X3 Cys) member 1 antibody
    • small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin) antibody
    • Small inducible cytokine subfamily D member 1 antibody
    • Small-inducible cytokine D1 antibody
    • X3CL1_HUMAN antibody
    see all


  • Anti-CX3CL1 antibody (ab25088) at 1/1000 dilution + mouse recombinant protein at 0.025 µg

    HRP conjugated goat anti-rabbit at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?

    Exposure time: 1 minute

    The gel running conditions were denaturing.

    The membrane was blocked with 10% milk for 30 minutes at RT and the primary antibody was incubated with the membrane for 16 hours at 4°C

    See Abreview

  • ab25088 (4µg/ml) staining CX3CL1 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasmic region of the cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab25088 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25088, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Senouthai S  et al. Fractalkine is Involved in Lipopolysaccharide-Induced Podocyte Injury through the Wnt/ß-Catenin Pathway in an Acute Kidney Injury Mouse Model. Inflammation N/A:N/A (2019). Read more (PubMed: 30919150) »
  • Liu P  et al. CX3CL1/fractalkine enhances prostate cancer spinal metastasis by activating the Src/FAK pathway. Int J Oncol 53:1544-1556 (2018). Read more (PubMed: 30066854) »
See all 30 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Western blot
Rat Cell lysate - whole cell (Primary astrocytes)
Loading amount
2 µg
Primary astrocytes
Gel Running Conditions
Reduced Denaturing (gel 10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 16 2013

Western blot
Human Cell lysate - whole cell (endothelial cell)
Loading amount
80000 cells
endothelial cell
Gel Running Conditions
Reduced Denaturing (gel 6%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 01 2012

Immunocytochemistry/ Immunofluorescence
Human Cell (endothelial cell)
endothelial cell
Yes - Triton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 16 2012

Western blot
Mouse Recombinant protein (recombinant protein)
Loading amount
0.025 µg
recombinant protein
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 16 2007

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