Key features and details
- Rabbit polyclonal to CX3CL1 (Biotin)
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-CX3CL1 antibody (Biotin)
See all CX3CL1 primary antibodies
DescriptionRabbit polyclonal to CX3CL1 (Biotin)
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Human
Highly pure (>98%) recombinant human CX3CL1
FormLyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
Purification notesab84280 was purified by affinity chromatography and then biotinylated. It is sterile filtered.
Our Abpromise guarantee covers the use of ab84280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.
Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human CX3CL1.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 42 kDa.
The detection limit for recombinant human CX3CL1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionThe soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.
Tissue specificitySmall intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the intercrine delta family.
modificationsA soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.
O-glycosylated with core 1 or possibly core 8 glycans.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- A 152E5.2 antibody
- AB030188 antibody
- ABCD 3 antibody
ab84280 has not yet been referenced specifically in any publications.