Key features and details
- Rabbit polyclonal to CX3CR1
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-CX3CR1 antibody
See all CX3CR1 primary antibodies
DescriptionRabbit polyclonal to CX3CR1
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
- WB - Rat brain lysate, Mouse heart lysate. IHC-P - Mouse lymph Tissue Flow Cyt: Mouse spleen tissue lysate
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Aqueous buffered solution
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab217291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 40 kDa).|
FunctionReceptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
Tissue specificityExpressed in lymphoid and neural tissues.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- Beta chemokine receptor-like 1 antibody
- C X3 C CKR 1 antibody
- C-X3-C CKR-1 antibody
Flow Cytometry of Mouse Spleen with ab217310 at 1/100
All lanes : Anti-CX3CR1 antibody (ab217291) at 1/200 dilution
Lane 1 : Rat brain lysate
Lane 2 : Mouse heart lysate
All lanes : Anti-Rabbit HRP conjugated antibody at 1/3000 dilution
Predicted band size: 40 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraformaldehyde fixed, paraffin embedded Mouse lymph tissue labeling CX3CR1 with ab217291 at 1/500 dilution.
ab217291 has not yet been referenced specifically in any publications.