Overview

  • Product name
  • Description
    Rabbit polyclonal to CX3CR1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CX3CR1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab64633.)

  • Positive control
    • This antibody gave a positive signal in the following Human Lysates: HeLa Whole Cell, SW480 Whole Cell, Human Small Intestine Tissue, JEG-3 Whole Cell, Y79 Whole Cell, HepG2 Whole Cell, Caco 2 Whole Cell

Properties

Applications

Our Abpromise guarantee covers the use of ab51668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40,50 kDa (predicted molecular weight: 40 kDa).Can be blocked with CX3CR1 peptide (ab8125).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
  • Tissue specificity
    Expressed in lymphoid and neural tissues.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta chemokine receptor-like 1 antibody
    • C X3 C CKR 1 antibody
    • C-X3-C CKR-1 antibody
    • CCRL1 antibody
    • Chemokine C X3 C motif receptor 1 antibody
    • CMK BRL 1 antibody
    • CMK-BRL-1 antibody
    • CMK-BRL1 antibody
    • CMKBLR1 antibody
    • CMKDR1 antibody
    • CX3C chemokine receptor 1 antibody
    • CX3C CKR1 antibody
    • CX3C1_HUMAN antibody
    • CX3CR1 antibody
    • Fractalkine receptor antibody
    • G-protein coupled receptor 13 antibody
    • GPR13 antibody
    • GPRV28 antibody
    • V28 antibody
    see all

Images

  • CX3CR1 was immunoprecipitated using 0.5mg JEG3 whole cell extract, 5µg of Rabbit polyclonal to CX3CR1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, JEG3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab51668.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 40kDa; CX3CR1

  • All lanes : Anti-CX3CR1 antibody (ab51668) at 1 µg

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : SW480 whole cell lysate (ab3957)
    Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 5 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 7 : Caco 2 whole cell lysate (ab3950)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 40,50 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.



    CX3CR1 has a predicted MW of 40 kDa and has 3 known isoforms (SwissProt). The 50 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to CX3CR1.
  • ICC/IF image of ab51668 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51668, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab51668 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

I am very pleased to hear you would like to accept our offer and test ab51668 in rhesus monkey. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rhesus monkey and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your inquiry.
The immunogen sequence is N-terminal and shares 90% sequence homology with Rhesus macaque.
I hope this information helps. Please contact us with any other questions.

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Answer

Thank you for your enquiry and your interest in our products. All of these 4 antibodies (ab8021, ab7200, ab7201, ab51668) recognize CX3CR1 and are suitable for Western blot application. However, ab7200 has been tested and characterized for detecting rat proteinonly - and not human. The immunogen used to raise this antibody was a recombinant and natural rat CX3CR1 and we do not have any data yet if ab7200 would recognize the human form. It seems that ab8021 is the most popular amongst these antibodies and there are several Abreviews and specific publications listed on the product datasheet. You may wish to take a careful look at it before making the final decision: https://www.abcam.com/cx3cr1-antibody-ab8021.html As you may be aware, RT-PCR data should be treated carefully since, there is no clear correlation between mRNA and protein levels and sometimes it is not easy to predict the protein expression levels from quantitative mRNA data. There are several publications available which delineate the technical boundaries of the approaches for quantitative analysis of protein expression and reveal that simple deduction from mRNA transcript analysis is insufficient. For this reason, I would suggest running a good positive control along with the samples on the same gel; for this purpose tissue lysate from human spleen can be applied. I hope this helps and if I can assist further, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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