Overview

  • Product name
  • Description
    Rabbit polyclonal to CX3CR1
  • Host species
    Rabbit
  • Specificity
    Recombinant and natural rat CX3CR1
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, IP, Neutralisingmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Recombinant amino terminal fragment (Rat).

Properties

Applications

Our Abpromise guarantee covers the use of ab7200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
IHC-Fr 1/100 - 1/500.
WB 1/1000 - 1/2000.
IP 1/300 - 1/800.
Neutralising Use at an assay dependent concentration.

Target

  • Function
    Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
  • Tissue specificity
    Expressed in lymphoid and neural tissues.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta chemokine receptor-like 1 antibody
    • C X3 C CKR 1 antibody
    • C-X3-C CKR-1 antibody
    • CCRL1 antibody
    • Chemokine C X3 C motif receptor 1 antibody
    • CMK BRL 1 antibody
    • CMK-BRL-1 antibody
    • CMK-BRL1 antibody
    • CMKBLR1 antibody
    • CMKDR1 antibody
    • CX3C chemokine receptor 1 antibody
    • CX3C CKR1 antibody
    • CX3C1_HUMAN antibody
    • CX3CR1 antibody
    • Fractalkine receptor antibody
    • G-protein coupled receptor 13 antibody
    • GPR13 antibody
    • GPRV28 antibody
    • V28 antibody
    see all

References

This product has been referenced in:
  • Wang L  et al. Aspirin modulates the inflammatory response in a thrombus-stimulated LMVEC model. Int J Mol Med 41:3253-3266 (2018). Read more (PubMed: 29568915) »
  • Gazerani S  et al. The Role of C Fibers in Spinal Microglia Induction and Possible Relation with TRPV3 Expression During Chronic Inflammatory Arthritis in Rats. Basic Clin Neurosci 7:231-40 (2016). Rat . Read more (PubMed: 27563416) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry and your interest in our products. All of these 4 antibodies (ab8021, ab7200, ab7201, ab51668) recognize CX3CR1 and are suitable for Western blot application. However, ab7200 has been tested and characterized for detecting rat proteinonly - and not human. The immunogen used to raise this antibody was a recombinant and natural rat CX3CR1 and we do not have any data yet if ab7200 would recognize the human form. It seems that ab8021 is the most popular amongst these antibodies and there are several Abreviews and specific publications listed on the product datasheet. You may wish to take a careful look at it before making the final decision: https://www.abcam.com/cx3cr1-antibody-ab8021.html As you may be aware, RT-PCR data should be treated carefully since, there is no clear correlation between mRNA and protein levels and sometimes it is not easy to predict the protein expression levels from quantitative mRNA data. There are several publications available which delineate the technical boundaries of the approaches for quantitative analysis of protein expression and reveal that simple deduction from mRNA transcript analysis is insufficient. For this reason, I would suggest running a good positive control along with the samples on the same gel; for this purpose tissue lysate from human spleen can be applied. I hope this helps and if I can assist further, please do not hesitate to contact me.

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Answer

Thank you for your enquiry. Yes, you are correct we have received a favourable Abreview using this antibody by western blotting where the antibody was diluted 1:1500 in 5% non-fat milk/PBST and the blots were labelled for two hours. This is supported by our recommended dilution range of WB: 1/1000 - 1/2000. We do not have any specific conditions for western blotting using this antibody. However, I would recommend our standard western blotting protocol which can be accessed at the following web link: https://www.abcam.com/index.html?pageconfig=view_protocol&pid=161 As detailed previously I would recommend using PBST, 5% non-fat milk and a two hour incubation. I would also recommend using a HRP conjugated secondary antibody and ECL plus as the method of detection as this is one of the most sensitive methods. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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