Anti-CX3CR1 antibody (ab7201)

Submit a review Q&A (2)References (7)

Overview

  • Product name
    Anti-CX3CR1 antibody
    See all CX3CR1 primary antibodies
  • Description
    Rabbit polyclonal to CX3CR1
  • Host species
    Rabbit
  • Specificity
    Recombinant and natural human and rat CX3CR1.
  • Tested applications
    Suitable for: IHC-Fr, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant amino terminal fragment (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab7201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100 - 1/500.
WB 1/1000 - 1/2000.
IP 1/300 - 1/800.
IHC-P Use at an assay dependent concentration. PubMed: 16452465

Target

  • Function
    Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
  • Tissue specificity
    Expressed in lymphoid and neural tissues.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • Beta chemokine receptor-like 1 antibody
      • C X3 C CKR 1 antibody
      • C-X3-C CKR-1 antibody
      • CCRL1 antibody
      • Chemokine C X3 C motif receptor 1 antibody
      • CMK BRL 1 antibody
      • CMK-BRL-1 antibody
      • CMK-BRL1 antibody
      • CMKBLR1 antibody
      • CMKDR1 antibody
      • CX3C chemokine receptor 1 antibody
      • CX3C CKR1 antibody
      • CX3C1_HUMAN antibody
      • CX3CR1 antibody
      • Fractalkine receptor antibody
      • G-protein coupled receptor 13 antibody
      • GPR13 antibody
      • GPRV28 antibody
      • V28 antibody
      see all

    Images

    • Western blot - Anti-CX3CR1 antibody (ab7201)
      Western blot - Anti-CX3CR1 antibody (ab7201)
      Western blot detection of CX3CR1 in the Brain by rabbit polyclonal to human CX3CR1 (V28). Western blot detection of CX3CR1 in the Brain by rabbit polyclonal to human CX3CR1 (V28).

    References

    This product has been referenced in:
    • Song CH  et al. Identification of chemoattractive factors involved in the migration of bone marrow-derived mesenchymal stem cells to brain lesions caused by prions. J Virol 85:11069-78 (2011). Read more (PubMed: 21813601) »
    • Jamieson WL  et al. CX3CR1 is expressed by prostate epithelial cells and androgens regulate the levels of CX3CL1/fractalkine in the bone marrow: potential role in prostate cancer bone tropism. Cancer Res 68:1715-22 (2008). Human . Read more (PubMed: 18339851) »
    See all 7 Publications for this product

    Publishing research using ab7201? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Hello I want to purchase a WB Ab for human CX3CR1 to verify that my siRNA works. Which of the 4 products you have is best to order for this application (ab8021, ab7200, ab7201, ab51668)? Thank you

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    Answer

    Thank you for your enquiry and your interest in our products. All of these 4 antibodies (ab8021, ab7200, ab7201, ab51668) recognize CX3CR1 and are suitable for Western blot application. However, ab7200 has been tested and characterized for detecting rat proteinonly - and not human. The immunogen used to raise this antibody was a recombinant and natural rat CX3CR1 and we do not have any data yet if ab7200 would recognize the human form. It seems that ab8021 is the most popular amongst these antibodies and there are several Abreviews and specific publications listed on the product datasheet. You may wish to take a careful look at it before making the final decision: https://www.abcam.com/cx3cr1-antibody-ab8021.html As you may be aware, RT-PCR data should be treated carefully since, there is no clear correlation between mRNA and protein levels and sometimes it is not easy to predict the protein expression levels from quantitative mRNA data. There are several publications available which delineate the technical boundaries of the approaches for quantitative analysis of protein expression and reveal that simple deduction from mRNA transcript analysis is insufficient. For this reason, I would suggest running a good positive control along with the samples on the same gel; for this purpose tissue lysate from human spleen can be applied. I hope this helps and if I can assist further, please do not hesitate to contact me.

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    Question

    I am looking to buy an antibody against mouse CX3CR1 to be used for IHC-Fr. Please can you advise whether either ab7201 has recently been tested on mouse or whether ab8020 has been used successfully for IHC-Fr.

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    Answer

    Thank you for your enquiry. As far as we are aware, cross reactivity with mouse has not yet been tested for use with ab7201. Also, ab8020 has not yet been tested for application in IHC-frozen sections (the antibody has only so far been tested in Western blotting). Should you decide to go ahead and purchase one of these products, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 points will be offered for an image). Please let me know if you require any further assistance.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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