Overview

  • Product name
  • Description
    Rabbit polyclonal to CX3CR1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to N terminal amino acids 2-21 of Human CX3CR1 (Peptide available as ab8125.)

  • Positive control
    • Human spleen tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab8021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/100.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration. Can be blocked with CX3CR1 peptide (ab8125).
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
  • Tissue specificity
    Expressed in lymphoid and neural tissues.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta chemokine receptor-like 1 antibody
    • C X3 C CKR 1 antibody
    • C-X3-C CKR-1 antibody
    • CCRL1 antibody
    • Chemokine C X3 C motif receptor 1 antibody
    • CMK BRL 1 antibody
    • CMK-BRL-1 antibody
    • CMK-BRL1 antibody
    • CMKBLR1 antibody
    • CMKDR1 antibody
    • CX3C chemokine receptor 1 antibody
    • CX3C CKR1 antibody
    • CX3C1_HUMAN antibody
    • CX3CR1 antibody
    • Fractalkine receptor antibody
    • G-protein coupled receptor 13 antibody
    • GPR13 antibody
    • GPRV28 antibody
    • V28 antibody
    see all

Images

  • Mouse heart tissue stained for CX3CR1 using ab8021 at 2 µg/ml in immunohistochemical analysis.

  • All lanes : Anti-CX3CR1 antibody (ab8021)

    Lane 1 : Human spleen lysate with absence of blocking peptide.
    Lane 2 : Human spleen lysate with presence of blocking peptide.

    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical staining (paraffin embedded) of human heart tissue using anti-CX3CR1 at 2 µg/ml.
  • Immunofluorescence of CX3CR1 in Human Heart cells using ab8021 at 10 ug/ml.

  • Flow cytometry analysis of THP-1 cells using CX3CR1 antibody (ab8021) at 0.1 ug/ml. 

    Green: Isotype control

    Red: CX3CR1 antibody (ab8021) 

  • Anti-CX3CR1 antibody (ab8021) at 1 µg/ml + Recombinant Human CX3CR1 protein (ab112276) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 4 minutes

References

This product has been referenced in:
  • Wang J  et al. Ischemia-induced Neuronal Cell Death Is Mediated by Chemokine Receptor CX3CR1. Sci Rep 8:556 (2018). Read more (PubMed: 29323156) »
  • Balzano T  et al. The Cerebellum of Patients with Steatohepatitis Shows Lymphocyte Infiltration, Microglial Activation and Loss of Purkinje and Granular Neurons. Sci Rep 8:3004 (2018). Read more (PubMed: 29445232) »
See all 37 Publications for this product

Customer reviews and Q&As

1-10 of 31 Abreviews or Q&A

Question
Answer

Thank you for contacting us. The alignment of the ab8021 immunogen sequence with the protein at the following link showed 90% identity, so we expect the antibody will react with your samples.

http://www.ncbi.nlm.nih.gov/protein/355559765?report=genbank&log$=protalign&blast_rank=20&RID=RTCPUT7M01N

The code we discussed will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rhesus macaque and include this code in the “Additional Comments” section so we know the Abreview is for this promotion.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Frozen Heart Sections (10um thick sections))
Permeabilization
Yes - 0.3% Trition for 30mins at RT
Specification
Frozen Heart Sections (10um thick sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 17 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Rat spinal cord with lesion)
Specification
Rat spinal cord with lesion
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 03 2015

Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Human Cultured Cells (human microglia cell induced from monocyte)
Specification
human microglia cell induced from monocyte
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 03 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Rat Tissue lysate - whole (Rat spinal cord with lesion on different days)
Specification
Rat spinal cord with lesion on different days
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jan 03 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 8,5
Sample
Human Tissue sections (Heart)
Specification
Heart
Permeabilization
Yes - Wash Buffer from Dako with Tween
Fixative
Paraformaldehyde

Mr. Rudolf Jung

Verified customer

Submitted May 28 2014

Application
Flow Cytometry
Fixation
Paraformaldehyde
Sample
Mouse Cell (BM)
Specification
BM
Gating Strategy
monocyte
Permeabilization
No

Abcam user community

Verified customer

Submitted Apr 25 2014

Answer

Thank you for contacting us about the issue you are having with the most recent vial. We have not had any complaints about lot GR82315-1 but if all your reagents and samples are the same as what you used for successful stains, then it is possible you received a a bad vial. We do have an alternative lot. Can you please tell me what your samples are (tissue, species, and fixation), how you block them before staining, and how much antibody you apply? What kind of antigen retrieval do you use, if any? Are you confident the secondary antibody is effective? Finally, do you know the lot number or numbers of the antibody that worked well?

Read More

Question
Answer

Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

This antibody was tested for flow cytometry in an outside laboratory, and all of the protocol details are not available. It was diluted 1:1000 for FC using CX3CR1-transfected cells, so the antibody may need to be used at a higher concentrated for non-transfected cells. We would recommend our standard blocking agent, 10% FCS, though other sera should be fine as well (except for rabbit serum, as this will results in high background from the anti-rabbit secondary antibody).

I am sorry that I don't have more information, but if you have further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

Read More

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. Your enquiry has been forwarded by #### to the scientific support team and we will be pleased to help you in this case.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for IHC on frozen and parafin embedded sections, and also mouse samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily. Once I have reviewed this, I hope to be able to provide further advice on the protocol.

I would appreciate if you could also provide some images which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.



Order Details
Antibody code:

Lot number:

Purchase order number
or preferably Abcam order number:


General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, low signal, non-specific satining etc.)

Sample (Species/Tissue/Cell Type/Cell Line etc.)


Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


Antigen retrieval (Enzymatic method, Heat mediated technique etc.)


Permeabilization step


Blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method


Positive and negative controls used (please specify)


Optimization attempts (problem solving)
How many times have you tried the IHC?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No


What steps have you altered?


Additional Notes


We would appreciate if you are also able to provide and image which woudl help us to assess the results

Read More

1-10 of 31 Abreviews or Q&A

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