Western blot abreview for Anti-CX3CR1 antibody

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Rat Tissue lysate - whole (Rat spinal cord with lesion on different days)
Specification
Rat spinal cord with lesion on different days
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA and 0.1% Tween 20 in TBS buffer
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Bands
Specific: 50 kDa
Exposure
1 minute(s) and 0 second(s)
Positive control
Rat spinal cord

Additional data

Additional Notes
Upon spinal cord lesion, the expression level of CX3CR1 is increased. GAPDH serves as the loading control.

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Submitted Jan 03 2015

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