Immunohistochemistry (Frozen sections) abreview for Anti-CX3CR1 antibody

Below Average
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in blocking (5% NGS/NDS in 0.3% PBST)
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546

Additional data

Additional Notes
there was a signal in the tissue after the staining, but it didn't look like microglia morphologically, and more importantly, it did not show co-localization with endogenous CX3CR1 mice or Iba1 antibody.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.

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Submitted May 15 2019

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