Key features and details
- Rabbit polyclonal to CX3CR1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CX3CR1 antibody
See all CX3CR1 primary antibodies
DescriptionRabbit polyclonal to CX3CR1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to Human CX3CR1 aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human spleen and lymph node tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab88577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 40 kDa).|
FunctionReceptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
Tissue specificityExpressed in lymphoid and neural tissues.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- Beta chemokine receptor-like 1 antibody
- C X3 C CKR 1 antibody
- C-X3-C CKR-1 antibody
All lanes : Anti-CX3CR1 antibody (ab88577) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Human lymph node tissue lysate - total protein (ab29871)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The predicted molecular weight of CX3CR1 is 40 kDa (SwissProt), however we expect to observe a banding pattern at 48 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab88577 has not yet been referenced specifically in any publications.