For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    cx3cr1-antibody-epr22354-15-ab245248.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Microbiology Interspecies Interaction Host Virus Interaction
Share by email
RecombinantRabMAb

Recombinant Anti-CX3CR1 antibody [EPR22354-15] (ab245248)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Flow Cytometry - Anti-CX3CR1 antibody (ab245248)
  • Flow Cytometry - Anti-CX3CR1 antibody (ab245248)
  • Flow Cytometry - Anti-CX3CR1 antibody (ab245248)
  • Flow Cytometry - Anti-CX3CR1 antibody (ab245248)
  • Anti-CX3CR1 antibody [EPR22354-15] (ab245248)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22354-15] to CX3CR1
  • Suitable for: Flow Cyt
  • Reacts with: Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CX3CR1 antibody [EPR22354-15]
    See all CX3CR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22354-15] to CX3CR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P,IP or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CX3CR1 aa 1-100. The exact sequence is proprietary.
    Database link: P49238

  • Positive control

    • Flow Cyt: Human peripheral blood mononuclear cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22354-15
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates
    • Immunology
    • Innate Immunity
    • Chemokines
    • Delta Chemokine Receptors
    • Neuroscience
    • Sensory System
    • Somatosensory system
    • Nociception
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related

Associated products

  • Alternative Versions

    • Anti-CX3CR1 antibody [EPR22354-15] - BSA and Azide free (ab267834)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Alexa Fluor® 488 Anti-CPSF6 antibody [EPR12898] (ab225248)

Applications

Our Abpromise guarantee covers the use of ab245248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/600.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P,IP or WB.
  • Target

    • Function

      Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
    • Tissue specificity

      Expressed in lymphoid and neural tissues.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P49238 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1524 Human
      • Omim: 601470 Human
      • SwissProt: P49238 Human
      • Unigene: 78913 Human
      • Alternative names

        • Beta chemokine receptor-like 1 antibody
        • C X3 C CKR 1 antibody
        • C-X3-C CKR-1 antibody
        • CCRL1 antibody
        • Chemokine C X3 C motif receptor 1 antibody
        • CMK BRL 1 antibody
        • CMK-BRL-1 antibody
        • CMK-BRL1 antibody
        • CMKBLR1 antibody
        • CMKDR1 antibody
        • CX3C chemokine receptor 1 antibody
        • CX3C CKR1 antibody
        • CX3C1_HUMAN antibody
        • CX3CR1 antibody
        • Fractalkine receptor antibody
        • G-protein coupled receptor 13 antibody
        • GPR13 antibody
        • GPRV28 antibody
        • V28 antibody
        see all

      Images

      • Flow Cytometry - Anti-CX3CR1 antibody (ab245248)
        Flow Cytometry - Anti-CX3CR1 antibody (ab245248)

        Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

        Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.

        Gated on viable cells and T cell panel (CD3+) is shown.

        Gated on viable cells and monocytes panel (CD14+) is shown.

      • Flow Cytometry - Anti-CX3CR1 antibody (ab245248)
        Flow Cytometry - Anti-CX3CR1 antibody (ab245248)

        Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

        Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.

        Negative control: CD19+ B cells. (PMID: 9390561).

        Gated on viable cells and B cell panel (CD19+) is shown.

      • Flow Cytometry - Anti-CX3CR1 antibody (ab245248)
        Flow Cytometry - Anti-CX3CR1 antibody (ab245248)

        Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

        Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.

        Gated on viable cells and NK cell panel (CD56+) is shown.

      • Flow Cytometry - Anti-CX3CR1 antibody (ab245248)
        Flow Cytometry - Anti-CX3CR1 antibody (ab245248)

        Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

        Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.

        Gated on viable cells and monocytes panel (CD14+) is shown.

      • Anti-CX3CR1 antibody [EPR22354-15] (ab245248)
        Anti-CX3CR1 antibody [EPR22354-15] (ab245248)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab245248? Please let us know so that we can cite the reference in this datasheet.

      ab245248 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab245248.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.