Recombinant Anti-CX3CR1 antibody [EPR22354-15] (ab245248)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CX3CR1 antibody [EPR22354-15]
See all CX3CR1 primary antibodies -
Description
Rabbit monoclonal [EPR22354-15] to CX3CR1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CX3CR1 aa 1-100. The exact sequence is proprietary.
Database link: P49238 -
Positive control
- FC: Human peripheral blood mononuclear cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22354-15 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab245248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/600. |
Target
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Function
Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1. -
Tissue specificity
Expressed in lymphoid and neural tissues. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1524 Human
- Omim: 601470 Human
- SwissProt: P49238 Human
- Unigene: 78913 Human
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Alternative names
- Beta chemokine receptor-like 1 antibody
- C X3 C CKR 1 antibody
- C-X3-C CKR-1 antibody
see all
Images
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Flow Cytometry - Anti-CX3CR1 antibody (ab245248)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.
Gated on viable cells and T cell panel (CD3+) is shown.
Gated on viable cells and monocytes panel (CD14+) is shown.
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Flow Cytometry - Anti-CX3CR1 antibody (ab245248)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.
Negative control: CD19+ B cells. (PMID: 9390561).
Gated on viable cells and B cell panel (CD19+) is shown.
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Flow Cytometry - Anti-CX3CR1 antibody (ab245248)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.
Gated on viable cells and NK cell panel (CD56+) is shown.
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Flow Cytometry - Anti-CX3CR1 antibody (ab245248)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.
Gated on viable cells and monocytes panel (CD14+) is shown.
Datasheets and documents
References
ab245248 has not yet been referenced specifically in any publications.
