Recombinant Anti-CX3CR1 antibody [EPR22354-15] (ab245248)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22354-15] to CX3CR1
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CX3CR1 antibody [EPR22354-15]
See all CX3CR1 primary antibodies -
Description
Rabbit monoclonal [EPR22354-15] to CX3CR1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CX3CR1 aa 1-100. The exact sequence is proprietary.
Database link: P49238 -
Positive control
- Flow Cyt: Human peripheral blood mononuclear cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22354-15 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab245248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/600. |
Target
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Function
Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1. -
Tissue specificity
Expressed in lymphoid and neural tissues. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1524 Human
- Omim: 601470 Human
- SwissProt: P49238 Human
- Unigene: 78913 Human
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Alternative names
- Beta chemokine receptor-like 1 antibody
- C X3 C CKR 1 antibody
- C-X3-C CKR-1 antibody
see all
Images
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Flow Cytometry - Anti-CX3CR1 antibody (ab245248)
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.
Gated on viable cells and T cell panel (CD3+) is shown.
Gated on viable cells and monocytes panel (CD14+) is shown.
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Flow Cytometry - Anti-CX3CR1 antibody (ab245248)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.
Negative control: CD19+ B cells. (PMID: 9390561).
Gated on viable cells and B cell panel (CD19+) is shown.
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Flow Cytometry - Anti-CX3CR1 antibody (ab245248)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.
Gated on viable cells and NK cell panel (CD56+) is shown.
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Flow Cytometry - Anti-CX3CR1 antibody (ab245248)Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.
Gated on viable cells and monocytes panel (CD14+) is shown.
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![Anti-CX3CR1 antibody [EPR22354-15] (ab245248)](https://www.abcam.com/ps/products/245/ab245248/Images/ab245248-9-benefits-of-recombinant-antibodies.png)
Anti-CX3CR1 antibody [EPR22354-15] (ab245248)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab245248 has not yet been referenced specifically in any publications.
