Overview

  • Product name
  • Description
    Sheep polyclonal to Cx40 / GJA5
  • Host species
    Sheep
  • Specificity
    Predicted not to cross react with Connexins 37, 43 or 45.
  • Tested applications
    Suitable for: ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Rat Cx40/ GJA5 aa 254-270.
    Sequence:

    SLVQGLTPPPDFNQCLK

  • Positive control
    • Cos-7 cells transfected with cDNA encoding GJA5. This antibody gave a positive result when used in the following methanol fixed cell lines: PC12
  • General notes

    Previously labelled as Connexin 40 / GJA5. 

Properties

Applications

Our Abpromise guarantee covers the use of ab16585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/10000 - 1/50000.
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • Involvement in disease
    Defects in GJA5 are a cause of familial atrial standstill (FAS) [MIM:108770]. Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm.
  • Sequence similarities
    Belongs to the connexin family. Alpha-type (group II) subfamily.
  • Cellular localization
    Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • Connexin 40 antibody
    • Connexin 40 / GJA5 antibody
    • Connexin-40 antibody
    • Cx40 antibody
    • CXA5_HUMAN antibody
    • Gap junction alpha 5 protein antibody
    • Gap junction alpha-5 protein antibody
    • Gap junction protein alpha 5 40kD (connexin 40) antibody
    • Gap junction protein alpha 5 antibody
    • Gja5 antibody
    • MGC11185 antibody
    see all

Images

  • ICC/IF image of ab16585 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab16585 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 donkey anti- Sheep (ab150177) IgG used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

References

This product has been referenced in:
  • Aschar-Sobbi R  et al. Increased atrial arrhythmia susceptibility induced by intense endurance exercise in mice requires TNFa. Nat Commun 6:6018 (2015). Read more (PubMed: 25598495) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain)
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 01 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (human umbilical vein endothelial cell)
Permeabilization
Yes - 0.5% TritonX-100
Specification
human umbilical vein endothelial cell
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 01 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (atrium)
Specification
atrium
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 25 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Permeabilization
Yes - triton-X 100
Specification
heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 23 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (right atrium, right ventricle and AV node and His)
Permeabilization
Yes - triton-X 100
Specification
right atrium, right ventricle and AV node and His
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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