Overview

  • Product name
  • Description
    Rabbit polyclonal to Cx40 / GJA5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Cx40/ GJA5 aa 102-134 (N terminal).
    Database link: P36382

  • Positive control
    • WB: Human placenta lysate. IHC-P: Human lung carcinoma tissue.
  • General notes

    Previously labelled as Connexin 40 / GJA5. 

Properties

Applications

Our Abpromise guarantee covers the use of ab38580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).

Target

  • Function
    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • Involvement in disease
    Defects in GJA5 are a cause of familial atrial standstill (FAS) [MIM:108770]. Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm.
  • Sequence similarities
    Belongs to the connexin family. Alpha-type (group II) subfamily.
  • Cellular localization
    Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • Connexin 40 antibody
    • Connexin 40 / GJA5 antibody
    • Connexin-40 antibody
    • Cx40 antibody
    • CXA5_HUMAN antibody
    • Gap junction alpha 5 protein antibody
    • Gap junction alpha-5 protein antibody
    • Gap junction protein alpha 5 40kD (connexin 40) antibody
    • Gap junction protein alpha 5 antibody
    • Gja5 antibody
    • MGC11185 antibody
    see all

Images

  • Anti-Cx40 / GJA5 antibody (ab38580) + Human placenta lysate

    Predicted band size: 40 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Cx40 / GJA5 with ab38580. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

  • ab38580 staining Cx40 / GJA5 in Mouse myocyte cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 10% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in serum) for 18 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG monoclonal (1/1000) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Zhang F  et al. Association between connexin 40 and potassium voltage-gated channel subfamily A member 5 expression in the atrial myocytes of patients with atrial fibrillation. Exp Ther Med 14:5170-5176 (2017). WB ; Human . Read more (PubMed: 29201233) »
  • Li S  et al. MicroRNA-208a-3p contributes to connexin40 remolding in human chronic atrial fibrillation. Exp Ther Med 14:5355-5362 (2017). Read more (PubMed: 29285063) »
See all 4 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (Fetal Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Fetal Liver
Blocking step
EnVision Flex Peroxidase-blocking reagent (ready-to-use - DAKO) as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde

Dr. Juliana Shimara Pires Ferrão

Verified customer

Submitted Nov 26 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (heart)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
heart
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 06 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (aglandular stomach, epithelium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
aglandular stomach, epithelium
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Methacarn

F Hernandez-Blazquez

Verified customer

Submitted Mar 17 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (myocyte)
Permeabilization
No
Specification
myocyte
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 13 2013

Question
Answer

Thank you for contacting us.

Lysates of cardiomyocytes can be used as positive control.

http://198.170.104.138/ijp/2005/44-54.pdf

We unfortunaltey do not have these lysates. Please check other vendors websites.

For more information about more lysates, please click the link below; http://www.proteinatlas.org/ENSG00000143140

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your email and for including the ICC images for your experiments with ab38580. Have you run a western blot to detect protein in your siRNA treated and untreated samples? Thanks!

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (heart)
Antigen retrieval step
None
Permeabilization
No
Specification
heart
Blocking step
Universalblocking reagent from biogenex as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Formaldehyde

Mrs. Sina Huntscha

Verified customer

Submitted Jan 31 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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