Overview

  • Product name

    Anti-CXCL11 antibody [EPR11076]
    See all CXCL11 primary antibodies
  • Description

    Rabbit monoclonal [EPR11076] to CXCL11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CXCL11 aa 1 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O14625

  • Positive control

    • WB: Human fetal spleen and THP1 lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 10 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
    • Tissue specificity

      High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
    • Sequence similarities

      Belongs to the intercrine alpha (chemokine CxC) family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • b R1 antibody
      • b-R1 antibody
      • Beta-R1 antibody
      • betaR1 antibody
      • C-X-C motif chemokine 11 antibody
      • Chemokine (C-X-C motif) ligand 11 antibody
      • Chemokine C-X-C motif ligand 11 antibody
      • CXC11 antibody
      • CXCL11 antibody
      • CXL11_HUMAN antibody
      • H174 antibody
      • I TAC antibody
      • I-TAC antibody
      • Interferon gamma inducible protein 9 antibody
      • Interferon gamma-inducible protein 9 antibody
      • Interferon inducible T cell alpha chemoattractant antibody
      • Interferon-inducible T-cell a chemoattractant I-TAC antibody
      • Interferon-inducible T-cell alpha chemoattractant antibody
      • IP 9 antibody
      • IP-9 antibody
      • IP9 antibody
      • ITAC antibody
      • MGC102770 antibody
      • SCYB11 antibody
      • SCYB9B antibody
      • Small inducible cytokine B11 antibody
      • Small inducible cytokine subfamily B (Cys-X-Cys) member 11 antibody
      • Small inducible cytokine subfamily B (Cys-X-Cys) member 9B antibody
      • Small inducible cytokine subfamily B, member 11 antibody
      • Small inducible cytokine subfamily B, member 9B antibody
      • Small-inducible cytokine B11 antibody
      see all

    Images

    • All lanes : Anti-CXCL11 antibody [EPR11076] (ab181035) at 1/1000 dilution (Purified)

      Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates prepared in RIPA lysis method
      Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates prepared in 1%SDS Hot lysis method

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 10 kDa
      Observed band size: 14 kDa
      why is the actual band size different from the predicted?



      We recommend to use 1%SDS Hot lysis prepararation method.

      For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    • Anti-CXCL11 antibody [EPR11076] (ab181035) at 1/5000 dilution (Purified) + Human fetal spleen lysates prepared in RIPA lysis method at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 10 kDa
      Observed band size: 14 kDa why is the actual band size different from the predicted?

    • All lanes : Anti-CXCL11 antibody [EPR11076] (ab181035) at 1/1000 dilution (Unpurified)

      Lane 1 : Human spleen lysate
      Lane 2 : THP1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP-labeled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 10 kDa

    References

    ab181035 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab181035.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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