Overview

  • Product name

    Anti-CXCL11 antibody [EPR21755-173]
    See all CXCL11 primary antibodies
  • Description

    Rabbit monoclonal [EPR21755-173] to CXCL11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CXCL11 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: O14625

  • Positive control

    • WB: Human CXCL11 recombinant protein (aa22-94); THP-1 treated with 200 ng/ml interferon-gamma (ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, followed by 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate. IP: THP-1 treated with 200 ng/ml interferon-gamma (ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, followed by 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab216157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 10 kDa.
IP 1/30.

Target

  • Function

    Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.
  • Tissue specificity

    High levels in peripheral blood leukocytes, pancreas and liver astrocytes. Moderate levels in thymus, spleen and lung. Low levels in placenta, prostate and small intestine. Also found in epidermal basal layer keratinocytes in skin disorders.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • b R1 antibody
    • b-R1 antibody
    • Beta-R1 antibody
    • betaR1 antibody
    • C-X-C motif chemokine 11 antibody
    • Chemokine (C-X-C motif) ligand 11 antibody
    • Chemokine C-X-C motif ligand 11 antibody
    • CXC11 antibody
    • CXCL11 antibody
    • CXL11_HUMAN antibody
    • H174 antibody
    • I TAC antibody
    • I-TAC antibody
    • Interferon gamma inducible protein 9 antibody
    • Interferon gamma-inducible protein 9 antibody
    • Interferon inducible T cell alpha chemoattractant antibody
    • Interferon-inducible T-cell a chemoattractant I-TAC antibody
    • Interferon-inducible T-cell alpha chemoattractant antibody
    • IP 9 antibody
    • IP-9 antibody
    • IP9 antibody
    • ITAC antibody
    • MGC102770 antibody
    • SCYB11 antibody
    • SCYB9B antibody
    • Small inducible cytokine B11 antibody
    • Small inducible cytokine subfamily B (Cys-X-Cys) member 11 antibody
    • Small inducible cytokine subfamily B (Cys-X-Cys) member 9B antibody
    • Small inducible cytokine subfamily B, member 11 antibody
    • Small inducible cytokine subfamily B, member 9B antibody
    • Small-inducible cytokine B11 antibody
    see all

Images

  • Anti-CXCL11 antibody [EPR21755-173] (ab216157) at 1/1000 dilution + Human CXCL11 recombinant protein (aa 22-94) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 10 kDa
    Observed band size: 9 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution: 5% NFDM/TBST

     

  • All lanes : Anti-CXCL11 antibody [EPR21755-173] (ab216157) at 1/1000 dilution

    Lane 1 : THP-1 (hman monocytic leukemia cell line) whole cell lysate
    Lane 2 : THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then 300 ng/ml Brefeldin A (BFA) was added to the treated cells for 20 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 10 kDa
    Observed band size: 11 kDa why is the actual band size different from the predicted?


    Exposure time: 37 seconds


    Blocking/Dilution: 5% NFDM/TBST

    The expression profile observed is consistent with what has been described in the literature (PMID: 17142784).

  • CXCL11 was immunoprecipitated from 0.35mg of THP-1 (human monocytic leukemia cell line) whole cell lysate with ab216157 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216157 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5,000 dilution.

    Lane 1: THP-1 (human monocytic leukemia cell line) treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate 10ug (Input).
    Lane 2: ab216157 IP in THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216157 in THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST

References

ab216157 has not yet been referenced specifically in any publications.

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