Anti-CXCL14 antibody (ab36622)

Reviews (2)Q&A (1)References (2)

Overview

  • Product name

    Anti-CXCL14 antibody
    See all CXCL14 primary antibodies

  • Description

    Rabbit polyclonal to CXCL14

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-FoFr, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Cow, Pig

  • Immunogen

    Synthetic peptide corresponding to Human CXCL14 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab36621)

  • Positive control

    • Human Cxcl14/BRAK recombinant protein

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 22815815
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).

Target

  • Function

    Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactive for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.

  • Tissue specificity

    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes.

  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • 1110031L23Rik antibody
      • 1200006I23Rik antibody
      • AI414372 antibody
      • BMAC antibody
      • bolekine antibody
      • BRAK antibody
      • Breast and kidney antibody
      • C-X-C motif chemokine 14 antibody
      • C-X-C motif chemokine ligand 14 antibody
      • Chaemokine, CXC motif, ligand 14 antibody
      • Chemokine (C-X-C motif) ligand 14 antibody
      • Chemokine BRAK antibody
      • CXC chemokine in breast and kidney antibody
      • CXCL14 antibody
      • CXL14_HUMAN antibody
      • JSC antibody
      • Kec antibody
      • Kidney-expressed chemokine CXC antibody
      • KS1 antibody
      • MGC10687 antibody
      • MGC124510 antibody
      • MGC90667 antibody
      • MIP 2 gamma antibody
      • MIP-2G antibody
      • MIP2G antibody
      • MIP2gamma antibody
      • NJAC antibody
      • PRO273 antibody
      • PSEC0212 antibody
      • Scyb14 antibody
      • Small Inducible Cytokine B14 antibody
      • Small inducible cytokine subfamily B (Cys-X-Cys) member 14 (BRAK) antibody
      • Small Inducible Cytokine subfamily B, member 14 antibody
      • Small-inducible cytokine B14 antibody
      • Tumor suppressing chemokine antibody
      • UNQ240 antibody
      see all

    Images

    • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-CXCL14 antibody (ab36622)
      Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-CXCL14 antibody (ab36622)Image from Strong JA et al., PLoS One. 2012;7(7):e40779. Epub 2012 Jul 16. Fig 5.; doi:10.1371/journal.pone.0040779; July 16, 2012, PLoS ONE 7(7): e40779.
      Immunohistochemical analysis of inflamed rat dorsal root ganglia, staining CXCL14 with ab36622.

      Dorsal root ganglia were taken 3 days after inflammation, from paraformaldehyde perfused rats and subsequently frozen. Sections were incubated with primary antibody at a dilution of 1/50 overnight at 4°C. An AlexaFluor®594-conjugated goat anti-rabbit IgG was used as the secondary antibody.
    • Western blot - Anti-CXCL14 antibody (ab36622)
      Western blot - Anti-CXCL14 antibody (ab36622)
      Anti-CXCL14 antibody (ab36622) at 1 µg/ml + Human Cxcl14/BRAK recombinant protein at 0.1 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 12 kDa
      Observed band size: 12 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL14 antibody (ab36622)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL14 antibody (ab36622)
      ab36622, at a 1/50 dilution, staining human CXCL14 in placenta tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

      See Abreview

    • Western blot - Anti-CXCL14 antibody (ab36622)
      Western blot - Anti-CXCL14 antibody (ab36622)

    References

    This product has been referenced in:

    • Schüler-Toprak S  et al. Agonists and knockdown of estrogen receptor ß differentially affect invasion of triple-negative breast cancer cells in vitro. BMC Cancer 16:951 (2016). Read more (PubMed: 28003019) »
    • Strong JA  et al. Microarray analysis of rat sensory ganglia after local inflammation implicates novel cytokines in pain. PLoS One 7:e40779 (2012). IHC-FoFr ; Rat . Read more (PubMed: 22815815) »
    See all 2 Publications for this product

    Publishing research using ab36622? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    Several months ago I bought the CXCL14 antibody (ab36622). In your protocol you use Antigen retrieval solution (Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution), but you did not specify which one you use (since I found 5 different retrieval solutions). I would really appreciate if you send me this information.

    Read More
    Answer

    Thank you for contacting us.

    The protocol that you have referenced was actually provided to us by another customer so I do not have that specific information for you. However the Dako Target Retrieval Solution appears to be a modified citrate buffer pH6.1. This agrees with our standard antigen retrieval as we recommend a citrate buffer pH6.0.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CXCL14 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Placenta)
    Specification
    Placenta
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization
    No
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Read More

    Mr. Antibody Solutions

    Verified customer

    Submitted May 28 2010

    Western blot abreview for Anti-CXCL14 antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Cardiac whole homogenates)
    Loading amount
    20 µg
    Specification
    Cardiac whole homogenates
    Gel Running Conditions
    Reduced Denaturing (10.5)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 02 2009

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