Overview

  • Product name

  • Description

    Rabbit polyclonal to CXCL14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant hBRAK (human BRAK).

  • General notes

    The sterile filtered antibody solution was lyophilized from 0.5xPBS, pH7.4.

Properties

  • Form

    Lyophilised:Reconstitute 100µl sterile water to give a concentration 1 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab46010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml. Detection limit 0.2 - 0.4 ng/well.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa. Detection limit 1.5 - 3.0 ng/lane under reducing or non-reducting conditions.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactive for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.
  • Tissue specificity

    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1110031L23Rik antibody
    • 1200006I23Rik antibody
    • AI414372 antibody
    • BMAC antibody
    • bolekine antibody
    • BRAK antibody
    • Breast and kidney antibody
    • C-X-C motif chemokine 14 antibody
    • C-X-C motif chemokine ligand 14 antibody
    • Chaemokine, CXC motif, ligand 14 antibody
    • Chemokine (C-X-C motif) ligand 14 antibody
    • Chemokine BRAK antibody
    • CXC chemokine in breast and kidney antibody
    • CXCL14 antibody
    • CXL14_HUMAN antibody
    • JSC antibody
    • Kec antibody
    • Kidney-expressed chemokine CXC antibody
    • KS1 antibody
    • MGC10687 antibody
    • MGC124510 antibody
    • MGC90667 antibody
    • MIP 2 gamma antibody
    • MIP-2G antibody
    • MIP2G antibody
    • MIP2gamma antibody
    • NJAC antibody
    • PRO273 antibody
    • PSEC0212 antibody
    • Scyb14 antibody
    • Small Inducible Cytokine B14 antibody
    • Small inducible cytokine subfamily B (Cys-X-Cys) member 14 (BRAK) antibody
    • Small Inducible Cytokine subfamily B, member 14 antibody
    • Small-inducible cytokine B14 antibody
    • Tumor suppressing chemokine antibody
    • UNQ240 antibody
    see all

Images

  • ab46010 staining Cxcl14/BRAK in human breast invasive, ductal carcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

  • ab46010 staining Cxcl14/BRAK in human breast invasive, ductal carcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

  • ICC/IF image of ab46010 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46010 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Ji X  et al. CXCL14 and NOS1 expression in specimens from patients with stage I-IIIA nonsmall cell lung cancer after curative resection. Medicine (Baltimore) 97:e0101 (2018). IHC ; Human . Read more (PubMed: 29517684) »
  • Wang Y  et al. HIC1 deletion promotes breast cancer progression by activating tumor cell/fibroblast crosstalk. J Clin Invest 128:5235-5250 (2018). Read more (PubMed: 30204129) »
See all 7 Publications for this product

Customer reviews and Q&As

Answer

Thank you, the images all arrived. I think you are correct, the TIFF files were probably too big.

Have you been able to obtain good stains for other proteins using this protocol?

I think the protocol is correct as it is. A customer did find that an enzymatic antigen retrieval gave a good MHC II stain of human tissue using ab55152 but I am not sure that will help with your samples. We do not know which enzyme he used but if you want to try an enzymatic antigen retrieval, I suggest using a trypsin solution. I can send more information about this if you like.The review can be found here:

https://www.abcam.com/index.html?datasheet=55152&tab=abreviews&intabreviewid=25380

Other antibodies may give better stains, assuming the samples have detectable amounts of CXCL14 and MHC class II. It is unlikely, though, that the EXPOSE kit ab80437 will improve the results.

I will be happy to send free replacements. I recommend the mouse monoclonal ab128065 for detection of MHC class II:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=128065).

The image on the datasheet is of a stain that uses a different substrate than what you are using but the antibody will work with your reagents, assuming you have an anti-mouse IgG secondary antibody. If not, please reply and I will make a recommendation for a compatible secondary antibody.

For CXCL14, I recommend the rabbit polyclonal ab36622:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=36622).

If you are using biotinylated secondary antibodies, you may want to consider using an endogenous biotin blocking kit. Your negative controls slides appear to have high background, possibly from endogenous biotin, and this may obscure the signal from CXCL14 and MHC II. We have a product for this, ab3387:

Click here (or use the following: https://www.abcam.com/index.html?datasheet=3387).

Please let me know if you need more information, and if you want to try the two antibodies that I recommend.

Read More

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