Key features and details
- Rabbit polyclonal to CXCL14
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CXCL14 antibody
See all CXCL14 primary antibodies
DescriptionRabbit polyclonal to CXCL14
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Human
Highly pure (>98%) recombinant hBRAK (human BRAK).
Although some customers have been successful in IHC we no longer batch test in this application.The sterile filtered antibody solution was lyophilized from 0.5xPBS, pH7.4.
FormLyophilized:Reconstitute 100µl sterile water to give a concentration 1 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab46010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.
To detect CXCL14 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa. Detection limit 1.5 - 3.0 ng/lane under reducing or non-reducting conditions.|
FunctionPotent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactive for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.
Tissue specificityExpressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
- Information by UniProt
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab46010 has been referenced in 7 publications.
- Ji X et al. CXCL14 and NOS1 expression in specimens from patients with stage I-IIIA nonsmall cell lung cancer after curative resection. Medicine (Baltimore) 97:e0101 (2018). IHC ; Human . PubMed: 29517684
- Wang Y et al. HIC1 deletion promotes breast cancer progression by activating tumor cell/fibroblast crosstalk. J Clin Invest 128:5235-5250 (2018). PubMed: 30204129
- Alexiadis M et al. Transcriptomic analysis of stage 1 versus advanced adult granulosa cell tumors. Oncotarget 7:14207-19 (2016). IHC . PubMed: 26893359
- Riester M et al. Risk Prediction for Late-Stage Ovarian Cancer by Meta-analysis of 1525 Patient Samples. J Natl Cancer Inst 106:N/A (2014). IHC-P ; Human . PubMed: 24700803
- Wang W et al. Antitumor efficacy of C-X-C motif chemokine ligand 14 in hepatocellular carcinoma in vitro and in vivo. Cancer Sci 104:1523-31 (2013). PubMed: 24033560
- Leach RE et al. High throughput, cell type-specific analysis of key proteins in human endometrial biopsies of women from fertile and infertile couples. Hum Reprod 27:814-828 (2012). IHC-P ; Human . PubMed: 22215622
- Gu XL et al. Expression of CXCL14 and its anticancer role in breast cancer. Breast Cancer Res Treat 135:725-35 (2012). WB . PubMed: 22910931