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    cxcl14-antibody-epr22807-28-ab264467.pdf

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Immunology Innate Immunity Chemokines Alpha Chemokines (CXC)
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RecombinantRabMAb

Recombinant Anti-CXCL14 antibody [EPR22807-28] (ab264467)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
  • Immunocytochemistry/ Immunofluorescence - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
  • Flow Cytometry (Intracellular) - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
  • Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
  • Anti-CXCL14 antibody [EPR22807-28] (ab264467)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22807-28] to CXCL14
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
ELISA
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Human CXCL14 ELISA Kit (ab213771)

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Overview

  • Product name

    Anti-CXCL14 antibody [EPR22807-28]
    See all CXCL14 primary antibodies
  • Description

    Rabbit monoclonal [EPR22807-28] to CXCL14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293 transfected with CXCL14, Human skin, Rat skin, RAW 264.7, Mouse skin, Rat lung and Mouse lung lysates. ICC/IF: HEK-293T cells transfected with myc-tagged CXCL14 expression vector.Flow Cyt (intra): HEK-293T cells transfected with myc-tagged CXCL14 expression vector.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22807-28
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

Associated products

  • Alternative Versions

    • Anti-CXCL14 antibody [EPR22807-28] - BSA and Azide free (ab264476)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • Anti-CXCL14 antibody (ab36622)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab264467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 13 kDa).
ICC/IF
1/100.

 

 

Notes
Flow Cyt (Intra)
1/500.
WB
1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 13 kDa).
ICC/IF
1/100.

 

 

Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactive for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.
  • Tissue specificity

    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession O95715 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9547 Human
    • Entrez Gene: 57266 Mouse
    • Entrez Gene: 306748 Rat
    • Omim: 604186 Human
    • SwissProt: O95715 Human
    • SwissProt: Q9WUQ5 Mouse
    • Unigene: 483444 Human
    • Unigene: 30211 Mouse
    • Alternative names

      • 1110031L23Rik antibody
      • 1200006I23Rik antibody
      • AI414372 antibody
      • BMAC antibody
      • bolekine antibody
      • BRAK antibody
      • Breast and kidney antibody
      • C-X-C motif chemokine 14 antibody
      • C-X-C motif chemokine ligand 14 antibody
      • Chaemokine, CXC motif, ligand 14 antibody
      • Chemokine (C-X-C motif) ligand 14 antibody
      • Chemokine BRAK antibody
      • CXC chemokine in breast and kidney antibody
      • CXCL14 antibody
      • CXL14_HUMAN antibody
      • JSC antibody
      • Kec antibody
      • Kidney-expressed chemokine CXC antibody
      • KS1 antibody
      • MGC10687 antibody
      • MGC124510 antibody
      • MGC90667 antibody
      • MIP 2 gamma antibody
      • MIP-2G antibody
      • MIP2G antibody
      • MIP2gamma antibody
      • NJAC antibody
      • PRO273 antibody
      • PSEC0212 antibody
      • Scyb14 antibody
      • Small Inducible Cytokine B14 antibody
      • Small inducible cytokine subfamily B (Cys-X-Cys) member 14 (BRAK) antibody
      • Small Inducible Cytokine subfamily B, member 14 antibody
      • Small-inducible cytokine B14 antibody
      • Tumor suppressing chemokine antibody
      • UNQ240 antibody
      see all

    Images

    • Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
      Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
      All lanes : Anti-CXCL14 antibody [EPR22807-28] (ab264467) at 1/1000 dilution

      Lane 1 : Human skin lysate at 10 µg
      Lane 2 : Rat skin lysate at 10 µg
      Lane 3 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
      Lane 4 : Mouse skin lysate at 20 µg
      Lane 5 : Rat lung lysate at 20 µg
      Lane 6 : Mouse lung lysate at 10 µg

      Secondary
      Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
      Lanes 3-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 13 kDa
      Observed band size: 14 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 15843547, 2602137, 28359053).

      This blot was developed using a higher sensitivity ECL substrate.

    • Immunocytochemistry/ Immunofluorescence - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
      Immunocytochemistry/ Immunofluorescence - Anti-CXCL14 antibody [EPR22807-28] (ab264467)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) (transfected with myc-tagged CXCL14 expression vector) cells labeling CXCL14 with ab264467 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary secondary antibody at 1/1000 dilution (Green). The myc tag is also detected with a Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) (Red). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged CXCL14 expression vector. The nuclear counter stain is DAPI (Blue).
      Secondary antibody only control: Used PBS instead of primary antibody, followed by  ab150077 AlexaFluor®488 Goat anti-Rabbit secondary secondary antibody at 1/1000 dilution.

    • Flow Cytometry (Intracellular) - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
      Flow Cytometry (Intracellular) - Anti-CXCL14 antibody [EPR22807-28] (ab264467)

      Intracellular flow cytometric analysis of [4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human embryonic kidney epithelial cell) (transfected with myc-tagged CXCL14 expression vector) cells labeling CXCL14 with ab264467 at 1/500 dilution (Right) compared with a Isotype control details (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150079), at 1/2000 dilution was used as the secondary antibody. 

      Cells were stained with rabbit IgG (Left) or ab264467 (Right). Then stained with anti-myc tag conjugated to Alexa Fluor® 488. 

       

       

    • Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
      Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
      All lanes : Anti-CXCL14 antibody [EPR22807-28] (ab264467) at 1/1000 dilution

      Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
      Lane 2 : HEK-293 transfected with CXCL14 (WT) expression vector containing a myc-His-tag®, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 13 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Anti-CXCL14 antibody [EPR22807-28] (ab264467)
      Anti-CXCL14 antibody [EPR22807-28] (ab264467)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab264467? Please let us know so that we can cite the reference in this datasheet.

    ab264467 has been referenced in 1 publication.

    • Diao LT  et al. N6-methyladenine demethylase ALKBH1 inhibits the differentiation of skeletal muscle. Exp Cell Res 400:112492 (2021). PubMed: 33529710

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