Recombinant Anti-CXCL14 antibody [EPR22807-28] (ab264467)
Overview
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Product name
Anti-CXCL14 antibody [EPR22807-28]
See all CXCL14 primary antibodies -
Description
Rabbit monoclonal [EPR22807-28] to CXCL14 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Mouse CXCL14 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q9WUQ5 -
Positive control
- WB: HEK-293 transfected with CXCL14, Human skin, Rat skin, RAW 264.7, Mouse skin, Rat lung and Mouse lung lysates. ICC/IF: HEK-293T cells transfected with myc-tagged CXCL14 expression vector. FC: HEK-293T cells transfected with myc-tagged CXCL14 expression vector.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22807-28 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab264467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 14 kDa (predicted molecular weight: 13 kDa). | |
| ICC/IF | 1/100.
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| Flow Cyt | 1/500.
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Target
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Function
Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactive for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. -
Tissue specificity
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highly expressed in normal tissue without inflammatory stimuli and infrequently expressed in cancer cell lines. Weakly expressed in monocyte-derived dendritic cells. Not detected in lung or unstimulated peripheral blood lymphocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9547 Human
- Entrez Gene: 57266 Mouse
- Entrez Gene: 306748 Rat
- Omim: 604186 Human
- SwissProt: O95715 Human
- SwissProt: Q9WUQ5 Mouse
- Unigene: 483444 Human
- Unigene: 30211 Mouse
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Alternative names
- 1110031L23Rik antibody
- 1200006I23Rik antibody
- AI414372 antibody
see all
Images
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![Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)](https://www.abcam.com/ps/products/264/ab264467/Images/ab264467-3-anti-cxcl14-antibody-epr22807-28-western-blot.jpg)
Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)All lanes : Anti-CXCL14 antibody [EPR22807-28] (ab264467) at 1/1000 dilution
Lane 1 : Human skin lysate at 10 µg
Lane 2 : Rat skin lysate at 10 µg
Lane 3 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 4 : Mouse skin lysate at 20 µg
Lane 5 : Rat lung lysate at 20 µg
Lane 6 : Mouse lung lysate at 10 µg
Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 3-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 13 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 15843547, 2602137, 28359053).
This blot was developed using a higher sensitivity ECL substrate.
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![Immunocytochemistry/ Immunofluorescence - Anti-CXCL14 antibody [EPR22807-28] (ab264467)](https://www.abcam.com/ps/products/264/ab264467/Images/ab264467-357007-anticxcl14-antibody-epr22807-28-immunocytochemistry-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CXCL14 antibody [EPR22807-28] (ab264467)Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) (transfected with myc-tagged CXCL14 expression vector) cells labeling CXCL14 with ab264467 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary secondary antibody at 1/1000 dilution (Green). The myc tag is also detected with a Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) (Red). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged CXCL14 expression vector. The nuclear counter stain is DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary secondary antibody at 1/1000 dilution. -
![Flow Cytometry - Anti-CXCL14 antibody [EPR22807-28] (ab264467)](https://www.abcam.com/ps/products/264/ab264467/Images/ab264467-1-anti-cxcl14-antibody-epr22807-28-flowcytometry-293t-overexpression-mouse.jpg)
Flow Cytometry - Anti-CXCL14 antibody [EPR22807-28] (ab264467)Flow cytometric analysis of [4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human embryonic kidney epithelial cell) (transfected with myc-tagged CXCL14 expression vector) cells labeling CXCL14 with ab264467 at 1/500 dilution (Right) compared with a Isotype control details (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150079), at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab264467 (Right). Then stained with anti-myc tag conjugated to Alexa Fluor® 488.
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![Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)](https://www.abcam.com/ps/products/264/ab264467/Images/ab264467-2-anti-cxcl14-antibody-epr22807-28-western-blot-hek293-transfected-with-cxcl14-human.jpg)
Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)All lanes : Anti-CXCL14 antibody [EPR22807-28] (ab264467) at 1/1000 dilution
Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293 transfected with CXCL14 (WT) expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 13 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References
ab264467 has not yet been referenced specifically in any publications.
