Overview

  • Product name

    Anti-CXCL15 antibody [EPR14470]
    See all CXCL15 primary antibodies
  • Description

    Rabbit monoclonal [EPR14470] to CXCL15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse CXCL15 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9WVL7

  • Positive control

    • WB: Mouse lung lysate. IHC-P: Mouse lung tissue. IP: Mouse lung lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14470
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/70.
WB 1/5000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

Target

  • Function

    Chemotactic for neutrophils. Involved in lung-specific neutrophil trafficking during normal and inflammatory conditions.
  • Tissue specificity

    Expression restricted to the lung, produced by bronchoepithelial cells and is released into the airways. Expressed at low levels in fetal lung.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • C-X-C motif chemokine 15 antibody
    • Chemokine (C-X-C motif) ligand 15 antibody
    • Cxcl15 antibody
    • CXL15_MOUSE antibody
    • Il8 antibody
    • Lungkine antibody
    • Scyb15 antibody
    • Small inducible cytokine B15 antibody
    • Small inducible cytokine subfamily B, member 15 antibody
    • Small-inducible cytokine B15 antibody
    • weche antibody
    see all

Images

  • All lanes : Anti-CXCL15 antibody [EPR14470] (ab197016) at 1/5000 dilution

    Lane 1 : Mouse lung tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Mouse heart tissue lysate
    Lane 4 : Mouse kidney tissue lysate
    Lane 5 : Mouse spleen tissue lysate
    Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CXCL15 with ab197016 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse lung tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CXCL15 with ab197016 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Negative on mouse kidney tissue. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • CXCL15 was immunoprecipitated from Mouse lung lysates with ab197016 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab197016 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Mouse lung lysate 10 µg (Input). Lane 2: ab197016 IP in Mouse lung lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab197016 in Mouse lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

ab197016 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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