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  1. Link

    cxcl1gro-alpha-antibody-epr19892-ab206411.pdf

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Immunology Innate Immunity Chemokines Alpha Chemokines (CXC)
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
  • Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
  • Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
  • Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19892] to CXCL1/GRO alpha
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-CXCL1/GRO alpha antibody [EPR19892]
    See all CXCL1/GRO alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR19892] to CXCL1/GRO alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human CXCL1/GRO alpha active protein; human GRO beta fragment recombinant protein; human GRO gamma fragment recombinant protein; human fetal lung lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19892
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-CXCL1/GRO alpha antibody [EPR19892] - BSA and Azide free (ab251456)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Recombinant mouse CXCL1/GRO alpha protein (ab202817)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab206411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/100. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
Notes
WB
1/100. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).

Target

  • Function

    Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P09341 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2919 Human
    • Omim: 155730 Human
    • SwissProt: P09341 Human
    • Unigene: 708652 Human
    • Unigene: 789 Human
    • Alternative names

      • C-X-C motif chemokine 1 antibody
      • Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antibody
      • chemokine (C-X-C motif) ligand 1 antibody
      • CINC-1 antibody
      • CXCL1 antibody
      • Cytokine-induced neutrophil chemoattractant 1 antibody
      • Fibroblast secretory protein antibody
      • Fsp antibody
      • Gro 1 antibody
      • Gro A antibody
      • Gro antibody
      • GRO protein, alpha antibody
      • GRO-alpha(1-73) antibody
      • GRO-alpha(6-73) antibody
      • Gro1 antibody
      • GRO1 oncogene (melanoma growth stimulating activity, alpha) antibody
      • GRO1 oncogene (melanoma growth-stimulating activity) antibody
      • Gro1 oncogene antibody
      • GROa antibody
      • GROA_HUMAN antibody
      • Growth-regulated alpha protein antibody
      • KC antibody
      • KC chemokine, mouse, homolog of antibody
      • melanoma growth stimulatory activity alpha antibody
      • Melanoma growth stimulatory activity antibody
      • Melanoma growth stimulatory activity, alpha antibody
      • MGSA alpha antibody
      • MGSA antibody
      • MGSA-a antibody
      • N51 antibody
      • NAP-3 antibody
      • NAP3 antibody
      • Neutrophil-activating protein 3 antibody
      • Platelet-derived growth factor-inducible protein KC antibody
      • Scyb 1 antibody
      • Scyb1 antibody
      • Secretory protein N51 antibody
      • Small inducible cytokine subfamily B, member 1 antibody
      see all

    Images

    • Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
      Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
      All lanes : Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411) at 1/100 dilution

      Lane 1 : Wild-type HeLa Treated TNF alpha (5 ng/mL, 6 h) + BFA (5 ug/mL, 6 h) cell lysate
      Lane 2 : CXCL1 knockout HeLa Treated TNF alpha (5 ng/mL, 6 h) + BFA (5 ug/mL, 6 h) cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 11 kDa
      Observed band size: 8 kDa why is the actual band size different from the predicted?



      False colour image of Western blot: Anti-CXCL1/GRO alpha antibody [EPR19892] staining at 1/100 dilution, shown in black; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab206411 was shown to bind specifically to CXCL1/GRO alpha. A band was observed at 8 kDa in treated wild-type HeLa cell lysates with no signal observed at this size in CXCL1 knockout cell line ab261774 (knockout cell lysate ab257079). To generate this image, wild-type and CXCL1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 20 minutes exposure time. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and HRP conjugated Goat anti-Mouse (H+L) at 1/20000 dilution.

    • Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
      Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
      All lanes : Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411) at 1/1000 dilution

      Lane 1 : Human CXCL1/GRO alpha active protein
      Lane 2 : Human GRO beta fragment recombinant protein
      Lane 3 : Human GRO gamma fragment recombinant protein

      Lysates/proteins at 0.01 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 11 kDa
      Observed band size: 10,36 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Human GRO beta fragment recombinant protein contain aa35-107 with a GST-Tag. Human GRO gamma fragment recombinant protein contain aa35-107 with a His-Tag®. These two protein were made in house.

    • Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
      Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
      Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411) at 1/100 dilution + Human fetal lung lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 11 kDa
      Observed band size: 11 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
      Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab206411? Please let us know so that we can cite the reference in this datasheet.

    ab206411 has been referenced in 1 publication.

    • Wang F  et al. Bioinformatics, Molecular Docking and Experiments In Vitro Analyze the Prognostic Value of CXC Chemokines in Breast Cancer. Front Oncol 11:665080 (2021). PubMed: 34123826

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