Anti-CXCL2 antibody (ab91511)
Key features and details
- Goat polyclonal to CXCL2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CXCL2 antibody
See all CXCL2 primary antibodies -
Description
Goat polyclonal to CXCL2 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Synthetic peptide corresponding to Human CXCL2 aa 94-107 (C terminal). Synthetic peptide:
C-KKIIEKMLKNGKSN
, corresponding to C terminal amino acids 94-107 of Human GRO beta (NP_002080.1).
Sequence:C-KKIIEKMLKNGKSN
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Positive control
- Recombinant Human CXCL2 protein (ab84204) can be used as a positive control in WB. Human duodenum lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab91511 was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 11 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 11 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsThe N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2920 Human
- Omim: 139110 Human
- SwissProt: P19875 Human
- Unigene: 75765 Human
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Alternative names
- C-X-C motif chemokine 2 antibody
- Chemokine (C X C motif) ligand 2 antibody
- Chemokine, CXC motif, ligand 2 antibody
see all
Images
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Anti-CXCL2 antibody (ab91511) at 0.2 µg/ml + Human duodenum lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour -
ab91511 (0.03µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Datasheets and documents
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Datasheet download
References (6)
ab91511 has been referenced in 6 publications.
- Kim J et al. Single-cell RNA sequencing for the identification of early-stage lung cancer biomarkers from circulating blood. NPJ Genom Med 6:87 (2021). PubMed: 34654834
- Yang J et al. Characteristics of CXCL2 expression in coronary atherosclerosis and negative regulation by microRNA-421. J Int Med Res 48:300060519896150 (2020). PubMed: 32212883
- Wilkinson HN et al. Elevated Local Senescence in Diabetic Wound Healing Is Linked to Pathological Repair via CXCR2. J Invest Dermatol 139:1171-1181.e6 (2019). PubMed: 30684552
- Wan XY et al. Antimetastatic effects of norcantharidin on hepatocellular carcinoma cells by up-regulating FAM46C expression. Am J Transl Res 9:155-166 (2017). WB ; Human . PubMed: 28123642
- Buoli Comani G et al. miRNA-regulated gene expression differs in celiac disease patients according to the age of presentation. Genes Nutr 10:482 (2015). PubMed: 26233308
- Magni S et al. miRNAs Affect the Expression of Innate and Adaptive Immunity Proteins in Celiac Disease. Am J Gastroenterol N/A:N/A (2014). PubMed: 25070052