Key features and details
- Rabbit polyclonal to CXCL2 (Biotin)
- Suitable for: WB, Sandwich ELISA
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-CXCL2 antibody (Biotin)
See all CXCL2 primary antibodies
DescriptionRabbit polyclonal to CXCL2 (Biotin)
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Predicted to work with: Human
- WB: Recombinant human CXCL2 protein. sELISA: Recombinant human CXCL2 protein.
FormLyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab245843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa.|
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.|
FunctionProduced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsThe N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells.
- Information by UniProt
- C-X-C motif chemokine 2 antibody
- Chemokine (C X C motif) ligand 2 antibody
- Chemokine, CXC motif, ligand 2 antibody
To detect human CXCL2 by Western Blot analysis ab245843 can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant human CXCL2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker.
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human CXCL2, respectively.
To detect human CXCL2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab245843 is required.
This biotinylated polyclonal antibody, in conjunction with a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human CXCL2.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245843 has not yet been referenced specifically in any publications.