Anti-CXCL5 antibody (ab9802)
Key features and details
- Rabbit polyclonal to CXCL5
- Suitable for: Sandwich ELISA
- Reacts with: Human
- Isotype: unknown
Overview
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Product name
Anti-CXCL5 antibody
See all CXCL5 primary antibodies -
Description
Rabbit polyclonal to CXCL5 -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Highly pure (>98%) recombinant hENA-78 (Human Epithelial Neutrophil Activating Peptide-78).
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General notes
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
To detect CXCL5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271281 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant CXCL5. |
| Notes |
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Sandwich ELISA
Use at an assay dependent concentration. To detect CXCL5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271281 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant CXCL5. |
Target
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Function
Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6374 Human
- Omim: 600324 Human
- SwissProt: P42830 Human
- Unigene: 89714 Human
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Alternative names
- AMCFII antibody
- C-X-C motif chemokine 5 antibody
- C-X-C motif chemokine ligand 5 antibody
see all
Images
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Sandwich ELISA - Anti-CXCL5 antibody (ab9802)
ab9802 detecting CXCL5 in sandwich ELISA
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab9802 has been referenced in 7 publications.
- Ando Y et al. Necroptosis in pancreatic cancer promotes cancer cell migration and invasion by release of CXCL5. PLoS One 15:e0228015 (2020). PubMed: 31999765
- Chen P et al. Symbiotic Macrophage-Glioma Cell Interactions Reveal Synthetic Lethality in PTEN-Null Glioma. Cancer Cell 35:868-884.e6 (2019). PubMed: 31185211
- Chen W et al. Taraxacum officinale extract ameliorates dextran sodium sulphate-induced colitis by regulating fatty acid degradation and microbial dysbiosis. J Cell Mol Med 23:8161-8172 (2019). PubMed: 31565850
- Han LL et al. miR-103 promotes the metastasis and EMT of hepatocellular carcinoma by directly inhibiting LATS2. Int J Oncol 53:2433-2444 (2018). PubMed: 30272278
- Hsu YL et al. Breast tumor-associated osteoblast-derived CXCL5 increases cancer progression by ERK/MSK1/Elk-1/Snail signaling pathway. Oncogene : (2012). IP . PubMed: 23045282
- Wang Q et al. Mutant proteins as cancer-specific biomarkers. Proc Natl Acad Sci U S A 108:2444-9 (2011). IP ; Human . PubMed: 21248225
- Rajashekhar G et al. Divergent and convergent effects on gene expression and function in acute versus chronic endothelial activation. Physiol Genomics 31:104-13 (2007). WB ; Mouse . PubMed: 17566077