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    cxcl5-antibody-ab9802.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-CXCL5 antibody (ab9802)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (7)

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Sandwich ELISA - Anti-CXCL5 antibody (ab9802)

    Key features and details

    • Rabbit polyclonal to CXCL5
    • Suitable for: Sandwich ELISA
    • Reacts with: Human
    • Isotype: unknown

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    Overview

    • Product name

      Anti-CXCL5 antibody
      See all CXCL5 primary antibodies
    • Description

      Rabbit polyclonal to CXCL5
    • Host species

      Rabbit
    • Tested applications

      Suitable for: Sandwich ELISAmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Highly pure (>98%) recombinant hENA-78 (Human Epithelial Neutrophil Activating Peptide-78).

    • General notes

      Although some customers have been successful in IHC we no longer batch test in this application.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      No preservative, sterile filtered
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      unknown
    • Light chain type

      unknown
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Immunology
      • Innate Immunity
      • Chemokines
      • Alpha Chemokines (CXC)
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Leukocyte recruitment
      • Chemokines

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Positive Controls

      • Recombinant human CXCL5 protein (ab9803)
    • Recombinant Protein

      • Recombinant Mouse CXCL5 protein (ab50173)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab9802 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA
    Use at an assay dependent concentration.

    To detect CXCL5  by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271281 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant CXCL5.

    Notes
    Sandwich ELISA
    Use at an assay dependent concentration.

    To detect CXCL5  by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271281 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant CXCL5.

    Target

    • Function

      Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
    • Sequence similarities

      Belongs to the intercrine alpha (chemokine CxC) family.
    • Post-translational
      modifications

      N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P42830 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6374 Human
      • Omim: 600324 Human
      • SwissProt: P42830 Human
      • Unigene: 89714 Human
      • Alternative names

        • AMCFII antibody
        • C-X-C motif chemokine 5 antibody
        • C-X-C motif chemokine ligand 5 antibody
        • Chemokine (C X C motif) ligand 5 antibody
        • chemokine (C-X-C motif) ligand 5 antibody
        • Cxcl5 antibody
        • CXCL5_HUMAN antibody
        • ENA 78 antibody
        • ENA-78 (8-78) antibody
        • ENA-78(1-78) antibody
        • ENA-78(9-78) antibody
        • ENA78 antibody
        • Epithelial derived neutrophil activating protein 78 antibody
        • Epithelial-derived neutrophil-activating protein 78 antibody
        • Lipopolysaccharide-induced CXC chemokine antibody
        • Neutrophil activating peptide ENA 78 antibody
        • Neutrophil activating protein 78 antibody
        • Neutrophil-activating peptide ENA-78 antibody
        • neutrophil-activating protein 78 antibody
        • SCYB5 antibody
        • Small inducible cytokine B5 antibody
        • small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78) antibody
        • small inducible cytokine subfamily B, member 5 antibody
        • Small-inducible cytokine B5 antibody
        see all

      Images

      • Sandwich ELISA - Anti-CXCL5 antibody (ab9802)
        Sandwich ELISA - Anti-CXCL5 antibody (ab9802)

        ab9802 detecting CXCL5 in sandwich ELISA

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (7)

      Publishing research using ab9802? Please let us know so that we can cite the reference in this datasheet.

      ab9802 has been referenced in 7 publications.

      • Ando Y  et al. Necroptosis in pancreatic cancer promotes cancer cell migration and invasion by release of CXCL5. PLoS One 15:e0228015 (2020). PubMed: 31999765
      • Chen P  et al. Symbiotic Macrophage-Glioma Cell Interactions Reveal Synthetic Lethality in PTEN-Null Glioma. Cancer Cell 35:868-884.e6 (2019). PubMed: 31185211
      • Chen W  et al. Taraxacum officinale extract ameliorates dextran sodium sulphate-induced colitis by regulating fatty acid degradation and microbial dysbiosis. J Cell Mol Med 23:8161-8172 (2019). PubMed: 31565850
      • Han LL  et al. miR-103 promotes the metastasis and EMT of hepatocellular carcinoma by directly inhibiting LATS2. Int J Oncol 53:2433-2444 (2018). PubMed: 30272278
      • Hsu YL  et al. Breast tumor-associated osteoblast-derived CXCL5 increases cancer progression by ERK/MSK1/Elk-1/Snail signaling pathway. Oncogene : (2012). IP . PubMed: 23045282
      • Wang Q  et al. Mutant proteins as cancer-specific biomarkers. Proc Natl Acad Sci U S A 108:2444-9 (2011). IP ; Human . PubMed: 21248225
      • Rajashekhar G  et al. Divergent and convergent effects on gene expression and function in acute versus chronic endothelial activation. Physiol Genomics 31:104-13 (2007). WB ; Mouse . PubMed: 17566077

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunoprecipitation abreview for Anti-CXCL5 antibody

      Excellent
      Abreviews
      Abreviews
      Application
      Immunoprecipitation
      Sample
      Human Cell lysate - whole cell (human pancreas and colon)
      Total protein in input
      5000 µg
      Specification
      human pancreas and colon
      Immuno-precipitation step
      Protein G
      Read More

      Mr. Qing Wang

      Verified customer

      Submitted Aug 02 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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