Anti-CXCL5 antibody (ab9802)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-CXCL5 antibody
See all CXCL5 primary antibodies -
DescriptionRabbit polyclonal to CXCL5
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Host speciesRabbit
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Tested applicationsSuitable for: ELISA, Neutralising, WB, IP, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Highly pure (>98%) recombinant hENA-78 (Human Epithelial Neutrophil Activating Peptide-78).
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Positive control
- Recombinant human CXCL5 protein (ab9803) can be used as a positive control in WB. This antibody gave a positive result in IHC in the following FFPE tissue: Human pancreas adenocarcinoma.
Properties
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FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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Isotypeunknown
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab9802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use a concentration of 0.5 µg/ml. | |
| Neutralising | Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hENA-78 (100.0 ng/ml), a concentration of 15 - 20 µg/ml of this antibody is required. | |
| WB | Use a concentration of 0.1 - 0.2 µg/ml. | |
| IP | 1/2500. | |
| IHC-P | Use a concentration of 1 µg/ml. |
Target
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FunctionInvolved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
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Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
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Post-translational
modificationsN-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 6374 Human
- Omim: 600324 Human
- SwissProt: P42830 Human
- Unigene: 89714 Human
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Alternative names
- AMCFII antibody
- C-X-C motif chemokine 5 antibody
- C-X-C motif chemokine ligand 5 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL5 antibody (ab9802)IHC image of CXCL5 staining in Human pancreas adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9802, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
References
This product has been referenced in:
- Hsu YL et al. Breast tumor-associated osteoblast-derived CXCL5 increases cancer progression by ERK/MSK1/Elk-1/Snail signaling pathway. Oncogene : (2012). IP . Read more (PubMed: 23045282) »
- Wang Q et al. Mutant proteins as cancer-specific biomarkers. Proc Natl Acad Sci U S A 108:2444-9 (2011). IP ; Human . Read more (PubMed: 21248225) »
