Anti-CXCL5 antibody (ab9802)
Key features and details
- Rabbit polyclonal to CXCL5
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Isotype: unknown
Overview
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Product name
Anti-CXCL5 antibody
See all CXCL5 primary antibodies -
Description
Rabbit polyclonal to CXCL5 -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human CXCL5. Highly pure (>98%) recombinant hENA-78 (Human Epithelial Neutrophil Activating Peptide-78).
Database link: P42830 -
General notes
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
To detect CXCL5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271281 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant CXCL5. |
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WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa.
To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Notes |
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Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. To detect CXCL5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271281 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant CXCL5. |
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa. To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Target
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Function
Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6374 Human
- Omim: 600324 Human
- SwissProt: P42830 Human
- Unigene: 89714 Human
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Alternative names
- AMCFII antibody
- C-X-C motif chemokine 5 antibody
- C-X-C motif chemokine ligand 5 antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (10)
ab9802 has been referenced in 10 publications.
- Wu T et al. Targeting HIC1/TGF-β axis-shaped prostate cancer microenvironment restrains its progression. Cell Death Dis 13:624 (2022). PubMed: 35853880
- Zhang Y et al. CircCTNNA1 acts as a ceRNA for miR-363-3p to facilitate the progression of colorectal cancer by promoting CXCL5 expression. J Biol Res (Thessalon) 28:7 (2021). PubMed: 33640021
- Ando Y et al. Necroptosis in pancreatic cancer promotes cancer cell migration and invasion by release of CXCL5. PLoS One 15:e0228015 (2020). PubMed: 31999765
- Peng L et al. Long noncoding RNA LBX2-AS1-modulated miR-4766-5p regulates gastric cancer development through targeting CXCL5. Cancer Cell Int 20:497 (2020). PubMed: 33061849
- Chen W et al. Taraxacum officinale extract ameliorates dextran sodium sulphate-induced colitis by regulating fatty acid degradation and microbial dysbiosis. J Cell Mol Med 23:8161-8172 (2019). PubMed: 31565850
- Chen P et al. Symbiotic Macrophage-Glioma Cell Interactions Reveal Synthetic Lethality in PTEN-Null Glioma. Cancer Cell 35:868-884.e6 (2019). PubMed: 31185211
- Han LL et al. miR-103 promotes the metastasis and EMT of hepatocellular carcinoma by directly inhibiting LATS2. Int J Oncol 53:2433-2444 (2018). PubMed: 30272278
- Hsu YL et al. Breast tumor-associated osteoblast-derived CXCL5 increases cancer progression by ERK/MSK1/Elk-1/Snail signaling pathway. Oncogene : (2012). IP . PubMed: 23045282
- Wang Q et al. Mutant proteins as cancer-specific biomarkers. Proc Natl Acad Sci U S A 108:2444-9 (2011). IP ; Human . PubMed: 21248225
- Rajashekhar G et al. Divergent and convergent effects on gene expression and function in acute versus chronic endothelial activation. Physiol Genomics 31:104-13 (2007). WB ; Mouse . PubMed: 17566077