Key features and details
- Rabbit polyclonal to CXCL5
- Suitable for: WB, Sandwich ELISA
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-CXCL5 antibody
See all CXCL5 primary antibodies
DescriptionRabbit polyclonal to CXCL5
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Mouse
Highly pure (>98%) 93 amino acid recombinant mLIX (mouse Lipopolysaccharide-induced CXC chemokine)
FormLyophilized:Reconstitute with 200µl of sterile water.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
No preservative, sterile filtered
Concentration information loading...
PurityImmunogen affinity purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab9983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.
The detection limit for recombinant mouse CXCL5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml.
Use 100 µl/well antibody solution. Detection limit of 0.2 - 0.4 ng/well of recombinant mouse CXCL5.
FunctionInvolved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsN-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
- Information by UniProt
- AMCFII antibody
- C-X-C motif chemokine 5 antibody
- C-X-C motif chemokine ligand 5 antibody
To detect mLIX by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine LIX (500-P146Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLIX.
All lanes : Anti-CXCL5 antibody (ab9983)
Lane 1 : Marker
Lane 2 : 250 ng
Lane 3 : 125 ng
Lane 4 : 62.5 ng
Lane 5 : 31.25 ng
Lane 6 : 15.625 ng
Lane 7 : 7.8 ng
Lane 8 : 3.9 ng
Lane 9 : 1.95 ng
Lane 10 : 0.975 ng
Lane 11 : 0.4875 ng
Lane 12 : 0.24 ng
ab9983 has been referenced in 2 publications.