Overview

  • Product name
    Anti-CXCL5 antibody (Biotin)
    See all CXCL5 primary antibodies
  • Description
    Rabbit polyclonal to CXCL5 (Biotin)
  • Host species
    Rabbit
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Sandwich ELISA, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CXCL5 aa 5-78.
    Sequence:

    SSRAAR VPGPSSSLCA LLVLLLLLTQ PGPIASAGPA AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKV


    Database link: P42830

  • Positive control
    • Recombinant Human CXCL5 protein.

Properties

  • Form
    Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    ab186670 was produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Human CXCL5 . ab186670 was purified by affinity chromatography and then biotinylated.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMCFII antibody
    • C-X-C motif chemokine 5 antibody
    • C-X-C motif chemokine ligand 5 antibody
    • chemokine (C-X-C motif) ligand 5 antibody
    • Cxcl5 antibody
    • CXCL5_HUMAN antibody
    • ENA 78 antibody
    • ENA-78 (8-78) antibody
    • ENA-78(1-78) antibody
    • ENA-78(9-78) antibody
    • ENA78 antibody
    • Epithelial derived neutrophil activating protein 78 antibody
    • Epithelial-derived neutrophil-activating protein 78 antibody
    • Lipopolysaccharide-induced CXC chemokine antibody
    • Neutrophil activating peptide ENA 78 antibody
    • Neutrophil-activating peptide ENA-78 antibody
    • neutrophil-activating protein 78 antibody
    • SCYB5 antibody
    • Small inducible cytokine B5 antibody
    • small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78) antibody
    • small inducible cytokine subfamily B, member 5 antibody
    • Small-inducible cytokine B5 antibody
    see all

Images

  • Direct  ELISA analysis of recombinant Human CXCL5 using ab186670 at a concentration of 0.25-1.0 μg/ml.
    ab186670, used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human CXCL5.

  • Sandwich ELISA analysis of recombinant Human CXCL5 using ab186670 at a concentration of 0.25-1.0 μg/ml.

    ab186670, used in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human CXCL5.

References

ab186670 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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