Product nameAnti-CXCL5 antibody (Biotin)
See all CXCL5 primary antibodies
DescriptionRabbit polyclonal to CXCL5 (Biotin)
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
- WB: Recombinant human CXCL5.
FormLyophilised:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.20
Constituents: PBS, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab271281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa.
Used in conjunction with compatible secondary reagents the detection limit for recombinant human CXCL5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|ELISA||Use a concentration of 0.25 - 1 µg/ml.|
FunctionInvolved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsN-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
- Information by UniProt
- AMCFII antibody
- C-X-C motif chemokine 5 antibody
- C-X-C motif chemokine ligand 5 antibody
Lane 1: Marker.
Lane 2: Recombinant hCXCL5 at 250ng/lane.
Lane 3: Recombinant hCXCL5 at 125ng/lane.
Lane 4: Recombinant hCXCL5 at 62.5ng/lane.
Lane 5: Recombinant hCXCL5 at 31.25ng/lane.
Lane 6: Recombinant hCXCL5 at 15.625ng/lane.
Lane 7: Recombinant hCXCL5 at 7.8ng/lane.
Lane 8: Recombinant hCXCL5 at 3.9ng/lane.
Lane 9: Recombinant hCXCL5 at 1.95ng/lane.
Lane 10: Recombinant hCXCL5 at 0.975ng/lane.
Lane 11: Recombinant hCXCL5 at 0.4875ng/lane.
Lane 12: Recombinant hCXCL5 at 0.24ng/lane.
Human ENA-78 (CXCL5) was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271281. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hENA-78 protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271281 has not yet been referenced specifically in any publications.