Recombinant
RabMAb

Anti-CXCL5 antibody [EPR4450(2)] (ab126763)

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Overview

  • Product name
    Anti-CXCL5 antibody [EPR4450(2)]
    See all CXCL5 primary antibodies
  • Description
    Rabbit monoclonal [EPR4450(2)] to CXCL5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CXCL5 aa 100 to the C-terminus.
    Database link: P42830

  • Positive control
    • A549 cell lysates treated with TNF alpha and TPA; Human CXCL5 recombinant protein.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 8 kDa (predicted molecular weight: 12 kDa).
IP 1/100 - 1/500.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function
      Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
    • Sequence similarities
      Belongs to the intercrine alpha (chemokine CxC) family.
    • Post-translational
      modifications
      N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
      • Alternative names
        • AMCFII antibody
        • C-X-C motif chemokine 5 antibody
        • C-X-C motif chemokine ligand 5 antibody
        • chemokine (C-X-C motif) ligand 5 antibody
        • Cxcl5 antibody
        • CXCL5_HUMAN antibody
        • ENA 78 antibody
        • ENA-78 (8-78) antibody
        • ENA-78(1-78) antibody
        • ENA-78(9-78) antibody
        • ENA78 antibody
        • Epithelial derived neutrophil activating protein 78 antibody
        • Epithelial-derived neutrophil-activating protein 78 antibody
        • Lipopolysaccharide-induced CXC chemokine antibody
        • Neutrophil activating peptide ENA 78 antibody
        • Neutrophil-activating peptide ENA-78 antibody
        • neutrophil-activating protein 78 antibody
        • SCYB5 antibody
        • Small inducible cytokine B5 antibody
        • small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78) antibody
        • small inducible cytokine subfamily B, member 5 antibody
        • Small-inducible cytokine B5 antibody
        see all

      Images

      • Western blot - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
        Western blot - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
        All lanes : Anti-CXCL5 antibody [EPR4450(2)] (ab126763) at 1/1000 dilution

        Lane 1 : A549 cell lysate,
        treated with TNF alpha and TPA at 10 µg
        Lane 2 : A549 cell lysate,
        untreated at 10 µg
        Lane 3 : Human CXCL5 recombinant protein at 0.001 µg

        Secondary
        All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

        Predicted band size: 12 kDa

      • Other - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
        Other - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab126763 has not yet been referenced specifically in any publications.

      Publishing research using ab126763? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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