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    cxcl5-antibody-epr44502-ab126763.pdf

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Immunology Innate Immunity Chemokines Alpha Chemokines (CXC)
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RecombinantRabMAb

Recombinant Anti-CXCL5 antibody [EPR4450(2)] (ab126763)

  • Datasheet
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Western blot - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
  • Immunoprecipitation - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
  • OI-RD Scanning - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
  • Anti-CXCL5 antibody [EPR4450(2)] (ab126763)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4450(2)] to CXCL5
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-CXCL5 antibody [EPR4450(2)]
    See all CXCL5 primary antibodies
  • Description

    Rabbit monoclonal [EPR4450(2)] to CXCL5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CXCL5 aa 100 to the C-terminus.
    Database link: P42830

  • Positive control

    • WB: A549 cell lysates treated with TNF alpha and TPA; Human CXCL5 recombinant protein. IP: A549 .
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 9.50 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4450(2)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-CXCL5 antibody [EPR4450(2)] - BSA and Azide free (ab248173)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 8 kDa (predicted molecular weight: 12 kDa).
IP
1/100 - 1/500.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 8 kDa (predicted molecular weight: 12 kDa).
IP
1/100 - 1/500.
Application notes
Is unsuitable for Flow Cyt or IHC-P.

Target

  • Function

    Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P42830 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6374 Human
    • Omim: 600324 Human
    • SwissProt: P42830 Human
    • Unigene: 89714 Human
    • Alternative names

      • AMCFII antibody
      • C-X-C motif chemokine 5 antibody
      • C-X-C motif chemokine ligand 5 antibody
      • Chemokine (C X C motif) ligand 5 antibody
      • chemokine (C-X-C motif) ligand 5 antibody
      • Cxcl5 antibody
      • CXCL5_HUMAN antibody
      • ENA 78 antibody
      • ENA-78 (8-78) antibody
      • ENA-78(1-78) antibody
      • ENA-78(9-78) antibody
      • ENA78 antibody
      • Epithelial derived neutrophil activating protein 78 antibody
      • Epithelial-derived neutrophil-activating protein 78 antibody
      • Lipopolysaccharide-induced CXC chemokine antibody
      • Neutrophil activating peptide ENA 78 antibody
      • Neutrophil activating protein 78 antibody
      • Neutrophil-activating peptide ENA-78 antibody
      • neutrophil-activating protein 78 antibody
      • SCYB5 antibody
      • Small inducible cytokine B5 antibody
      • small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78) antibody
      • small inducible cytokine subfamily B, member 5 antibody
      • Small-inducible cytokine B5 antibody
      see all

    Images

    • Western blot - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
      Western blot - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
      All lanes : Anti-CXCL5 antibody [EPR4450(2)] (ab126763) at 1/1000 dilution

      Lane 1 : A549 cell lysate,
      treated with TNF alpha and TPA at 10 µg
      Lane 2 : A549 cell lysate,
      untreated at 10 µg
      Lane 3 : Human CXCL5 recombinant protein at 0.001 µg

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 12 kDa

    • Immunoprecipitation - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
      Immunoprecipitation - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)

      CXCL5 was immunoprecipitated from 0.35 mg A549 (Human lung carcinoma epithelial cell) whole cell lysate 10 µg with 126763 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysate 10 µg

      Lane 2: ab126763 IP in A549 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab126763 in A549 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

    • OI-RD Scanning - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
      OI-RD Scanning - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
      Anti-CXCL5 antibody [EPR4450(2)] (ab126763)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab126763? Please let us know so that we can cite the reference in this datasheet.

    ab126763 has been referenced in 1 publication.

    • Luo M  et al. MicroRNA-432-5p inhibits cell migration and invasion by targeting CXCL5 in colorectal cancer. Exp Ther Med 21:301 (2021). PubMed: 33717244

    Customer reviews and Q&As

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