Recombinant Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
![Western blot - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)](https://www.abcam.com/ps/products/126/ab126763/Images/ab126763-140796-anti-cxcl5-antibody-epr44502-western-blot.jpg "Western blot - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4450(2)] to CXCL5
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CXCL5 antibody [EPR4450(2)]
See all CXCL5 primary antibodies -
Description
Rabbit monoclonal [EPR4450(2)] to CXCL5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CXCL5 aa 100 to the C-terminus.
Database link: P42830 -
Positive control
- WB: A549 cell lysates treated with TNF alpha and TPA; Human CXCL5 recombinant protein. IP: A549 .
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 9.50 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4450(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 8 kDa (predicted molecular weight: 12 kDa).
|
|
| IP |
1/100 - 1/500.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 8 kDa (predicted molecular weight: 12 kDa). |
|
IP
1/100 - 1/500. |
Target
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Function
Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6374 Human
- Omim: 600324 Human
- SwissProt: P42830 Human
- Unigene: 89714 Human
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Alternative names
- AMCFII antibody
- C-X-C motif chemokine 5 antibody
- C-X-C motif chemokine ligand 5 antibody
see all
Images
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Western blot - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)All lanes : Anti-CXCL5 antibody [EPR4450(2)] (ab126763) at 1/1000 dilution
Lane 1 : A549 cell lysate,
treated with TNF alpha and TPA at 10 µg
Lane 2 : A549 cell lysate,
untreated at 10 µg
Lane 3 : Human CXCL5 recombinant protein at 0.001 µg
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 12 kDa -
![Immunoprecipitation - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)](https://www.abcam.com/ps/products/126/ab126763/Images/ab126763-5-anti-cxcl5-antibody-epr4450-2-immunoprecipitation-a549-human.jpg)
Immunoprecipitation - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)CXCL5 was immunoprecipitated from 0.35 mg A549 (Human lung carcinoma epithelial cell) whole cell lysate 10 µg with 126763 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab126763 IP in A549 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab126763 in A549 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![OI-RD Scanning - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)](https://www.abcam.com/ps/products/126/ab126763/Images/ab126763-140795-anti-cxcl5-antibody-epr44502.jpg)
OI-RD Scanning - Anti-CXCL5 antibody [EPR4450(2)] (ab126763) -
![Anti-CXCL5 antibody [EPR4450(2)] (ab126763)](https://www.abcam.com/ps/products/126/ab126763/Images/ab126763-3-benefits-of-recombinant-antibodies.png)
Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab126763 has been referenced in 1 publication.
- Luo M et al. MicroRNA-432-5p inhibits cell migration and invasion by targeting CXCL5 in colorectal cancer. Exp Ther Med 21:301 (2021). PubMed: 33717244
