Recombinant Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
![Western blot - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)](https://www.abcam.com/ps/products/126/ab126763/Images/ab126763-140796-anti-cxcl5-antibody-epr44502-western-blot.jpg "Western blot - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4450(2)] to CXCL5
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-CXCL5 antibody [EPR4450(2)]
See all CXCL5 primary antibodies -
Description
Rabbit monoclonal [EPR4450(2)] to CXCL5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CXCL5 aa 100 to the C-terminus.
Database link: P42830 -
Positive control
- A549 cell lysates treated with TNF alpha and TPA; Human CXCL5 recombinant protein.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 9.50 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4450(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab126763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 8 kDa (predicted molecular weight: 12 kDa). | |
| IP | 1/100 - 1/500. |
Target
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Function
Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6374 Human
- Omim: 600324 Human
- SwissProt: P42830 Human
- Unigene: 89714 Human
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Alternative names
- AMCFII antibody
- C-X-C motif chemokine 5 antibody
- C-X-C motif chemokine ligand 5 antibody
see all
Images
-
Western blot - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)All lanes : Anti-CXCL5 antibody [EPR4450(2)] (ab126763) at 1/1000 dilution
Lane 1 : A549 cell lysate,
treated with TNF alpha and TPA at 10 µg
Lane 2 : A549 cell lysate,
untreated at 10 µg
Lane 3 : Human CXCL5 recombinant protein at 0.001 µg
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 12 kDa -
![OI-RD Scanning - Anti-CXCL5 antibody [EPR4450(2)] (ab126763)](https://www.abcam.com/ps/products/126/ab126763/Images/ab126763-140795-anti-cxcl5-antibody-epr44502.jpg)
OI-RD Scanning - Anti-CXCL5 antibody [EPR4450(2)] (ab126763) -
![Anti-CXCL5 antibody [EPR4450(2)] (ab126763)](https://www.abcam.com/ps/products/126/ab126763/Images/ab126763-3-benefits-of-recombinant-antibodies.png)
Anti-CXCL5 antibody [EPR4450(2)] (ab126763)
Protocols
Datasheets and documents
References (0)
ab126763 has not yet been referenced specifically in any publications.
