Recombinant Anti-CXCL7/PBP antibody [EPR19780] (ab206966)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CXCL7/PBP antibody [EPR19780]
See all CXCL7/PBP primary antibodies -
Description
Rabbit monoclonal [EPR19780] to CXCL7/PBP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human CXCL7/PBP aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: P02775 -
Positive control
- WB: Human NAP2 active recombinant protein; human serum and human spleen lysate. IP: Human serum.
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General notes
Previously labelled as CXCL7.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19780 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab206966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 14 kDa). | |
| IP | 1/30. |
Target
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Function
LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsProteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)).
Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor.
NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils.
NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5473 Human
- Omim: 121010 Human
- SwissProt: P02775 Human
- Unigene: 2164 Human
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Alternative names
- B TG1 antibody
- Beta TG antibody
- Beta thromboglobulin antibody
see all
Images
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![Western blot - Anti-CXCL7/PBP antibody [EPR19780] (ab206966)](https://www.abcam.com/ps/products/206/ab206966/Images/ab206966-261139-anti-cxcl7-pbp-antibody-epr19780-western-blot.jpg)
Western blot - Anti-CXCL7/PBP antibody [EPR19780] (ab206966)Anti-CXCL7/PBP antibody [EPR19780] (ab206966) at 1/1000 dilution + Human NAP2 active recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds -
![Western blot - Anti-CXCL7/PBP antibody [EPR19780] (ab206966)](https://www.abcam.com/ps/products/206/ab206966/Images/ab206966-261135-anti-cxcl7-pbp-antibody-epr19780-western-blot.jpg)
Western blot - Anti-CXCL7/PBP antibody [EPR19780] (ab206966)All lanes : Anti-CXCL7/PBP antibody [EPR19780] (ab206966) at 1/1000 dilution
Lane 1 : Human serum at 20 µg
Lane 2 : Human spleen lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 14 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 10 seconds; Lane 2: 3 minutes.
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![Immunoprecipitation - Anti-CXCL7/PBP antibody [EPR19780] (ab206966)](https://www.abcam.com/ps/products/206/ab206966/Images/ab206966-261134-anti-cxcl7-pbp-antibody-epr19780-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CXCL7/PBP antibody [EPR19780] (ab206966)NAP2 was immunoprecipitated from 0.35 mg of Human serum with ab206966 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab206966 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Human serum 10µg (Input).
Lane 2: ab206966 IP in Human serum.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206966 in Human serum.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
The band at 50 kD is likely to be human IgG in input and rabbit IgG heavy chain in +ve and –ve lane, but this has not been tested.
References
ab206966 has not yet been referenced specifically in any publications.
