Overview

  • Product name

    Anti-CXCL7/PBP antibody [EPR20036]
    See all CXCL7/PBP primary antibodies
  • Description

    Rabbit monoclonal [EPR20036] to CXCL7/PBP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IHC-Fr, IPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein within Mouse CXCL7/PBP aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9EQI5

  • Positive control

    • WB: Mouse CXCL7/PBP active protein; Mouse spleen, platelet, plasma and serum lysates. IHC-P: Mouse spleen and lung tissues. IHC-Fr: Mouse spleen tissue. IP: Mouse spleen lysate.
  • General notes

    Previously labelled as CXCL7. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab206406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 7 kDa (predicted molecular weight: 14 kDa).
IHC-Fr 1/500.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20)

IP 1/40.

Target

  • Function

    LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    Proteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)).
    Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor.
    NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils.
    NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • B TG1 antibody
    • Beta TG antibody
    • Beta thromboglobulin antibody
    • Beta-TG antibody
    • C-X-C motif chemokine 7 antibody
    • Chemokine (C X C motif) ligand 7 antibody
    • Connective tissue activating peptide III antibody
    • CTAP 3 antibody
    • CTAP III antibody
    • CTAP-III antibody
    • CTAP-III(1-81) antibody
    • CTAP3 antibody
    • CTAPIII antibody
    • CXC chemokine ligand 7 antibody
    • CXCL 7 antibody
    • CXCL7 antibody
    • CXCL7_HUMAN antibody
    • LA PF 4 antibody
    • LA-PF4 antibody
    • LDGF antibody
    • Leukocyte derived growth factor antibody
    • Leukocyte-derived growth factor antibody
    • Low-affinity platelet factor IV antibody
    • Macrophage-derived growth factor antibody
    • MDGF antibody
    • NAP 2 antibody
    • NAP-2 antibody
    • NAP-2(1-63) antibody
    • NAP-2(1-66) antibody
    • NAP-2(73) antibody
    • NAP-2(74) antibody
    • Neutrophil activating peptide 2 antibody
    • Neutrophil-activating peptide 2(1-63) antibody
    • PBP antibody
    • Platelet basic protein antibody
    • PPBP antibody
    • Pro platelet basic protein (chemokine (C-X-C motif) ligand 7) antibody
    • Pro platelet basic protein antibody
    • SCYB7 antibody
    • Small inducible cytokine subfamily B member 7 antibody
    • Small-inducible cytokine B7 antibody
    • TC1 antibody
    • TC2 antibody
    • TGB antibody
    • TGB1 antibody
    • THBGB antibody
    • THBGB1 antibody
    • Thrombocidin 1 antibody
    • Thrombocidin 2 antibody
    • Thromboglobulin, beta-1 antibody
    see all

Images

  • Anti-CXCL7/PBP antibody [EPR20036] (ab206406) at 1/5000 dilution + Mouse CXCL7/PBP active protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa
    Observed band size: 7 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling CXCL7/PBP with ab206406 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on megakaryocytes and platelets of mouse spleen was observed. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CXCL7/PBP with ab206406 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on megakaryocytes and platelets of mouse spleen is observed [PMID:16391012]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Lanes 1-2 : Anti-CXCL7/PBP antibody [EPR20036] (ab206406) at 1/2000 dilution
    Lanes 3-4 : Anti-CXCL7/PBP antibody [EPR20036] (ab206406) at 1/10000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Mouse plasma
    Lane 3 : Mouse platelet lysate
    Lane 4 : Mouse serum

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 14 kDa
    Observed band size: 7 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1/2: 4 seconds; Lane 3/4: 1 second.

    The molecular weight is consistent with what has been described in the literature: PMID: 14673015.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CXCL7/PBP with ab206406 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on platelets of mouse lung is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • CXCL7/PBP was immunoprecipitated from 0.35 mg of Mouse spleen lysate with ab206406 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206406 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse spleen lysate, 10 μg (Input).

    Lane 2: ab206406 IP in Mouse spleen lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206406 in Mouse spleen lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

ab206406 has not yet been referenced specifically in any publications.

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