Anti-CXCR1 antibody (ab124344)

Submit a review Q&A (1)References (2)

Overview

  • Product name

    Anti-CXCR1 antibody
    See all CXCR1 primary antibodies

  • Description

    Rabbit polyclonal to CXCR1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide derived from the N terminus of Human CXCR1 (NP_000625).

  • Positive control

    • Human fetal skin lysate; Human fetal tonsil tissue.

Properties

  • Form

    Lyophilised:Reconstitute in 200:micro;l of sterile H2O.
    The lyophilized antibody is stable for at least one year when stored at -20ºC after reconstitution.

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function

    Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • C C antibody
      • C C CKR 1 antibody
      • C-X-C chemokine receptor type 1 antibody
      • CCCKR1 antibody
      • CD 181 antibody
      • CD128 antibody
      • CD181 antibody
      • CD181 antigen antibody
      • CDw128a antibody
      • Chemokine (C X C motif) receptor 1 antibody
      • Chemokine (C X C) receptor 1 antibody
      • Chemokine receptor 1 antibody
      • CKR 1 antibody
      • CMKAR1 antibody
      • CXC-R1 antibody
      • CXCR 1 antibody
      • CXCR-1 antibody
      • CXCR1 antibody
      • CXCR1_HUMAN antibody
      • High affinity interleukin 8 receptor A antibody
      • High affinity interleukin-8 receptor A antibody
      • IL 8 receptor type 1 antibody
      • IL 8R A antibody
      • IL-8 receptor type 1 antibody
      • IL-8R A antibody
      • IL8 receptor antibody
      • IL8 receptor type 1 antibody
      • IL8R1 antibody
      • IL8RA antibody
      • IL8RBA antibody
      • Interleukin 8 receptor alpha antibody
      • Interleukin 8 receptor type 1 antibody
      • Interleukin 8 receptor type A antibody
      see all

    Images

    • Western blot - Anti-CXCR1 antibody (ab124344)
      Western blot - Anti-CXCR1 antibody (ab124344)
      Anti-CXCR1 antibody (ab124344) at 1/500 dilution + Human fetal skin lysate.

      Predicted band size: 40 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR1 antibody (ab124344)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR1 antibody (ab124344)
      ab124344, at 1/100 dilution, staining CXCR1 in formalin-fixed, paraffin-embedded Human fetal tonsil tissue.

    References

    This product has been referenced in:

    • Ilgin C  et al. The evaluation of the distribution of CD133, CXCR1 and the tumor associated macrophages in different molecular subtypes of breast cancer. Histol Histopathol N/A:18139 (2019). Read more (PubMed: 31250425) »
    • Kokje VBC  et al. CXCL8 hyper-signaling in the aortic abdominal aneurysm. Cytokine 108:96-104 (2018). IHC-P ; Human . Read more (PubMed: 29587155) »
    See all 2 Publications for this product

    Publishing research using ab124344? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Phone call requesting information about the anti-CXCR1 antibody (ab124344).

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    Answer

    Thank you for contacting us last week and sorry for the delay in getting back to you.

    I now have some further information to share with you in regards to the Anti-CXCR1 antibody (ab124344). You requested information relating to if the antibody hadbeen used specifically with cancer tissue. I can confirm that this has not been performed byus in house and we have hadno reports fromour customers of thiseither.However, the antibody has beenused to stain human fetal tonsil tissue.

    I now have furtherinformation as tothe protocol used to obtain this staining (presented on the datasheet of ab124344).The tissue wasformalin fixed andparaffin embedded. Following mounting and deparaffinisation the tissue was treatedwith10 mM boilingcitrate buffer (pH6.0) for 15 minutes to unmask the epitope. TheCXCR1proteinwasthenstained usingthe primary antibodyin a 1/100 dilution overnight at4ºC followedby an HRP conjugated secondary antibody for the detection with DAB.

    Although this antibodyhas not been used in cancer tissue specifically,I can reassure you that if you intend to use this antibody in paraffin embeddedIHCwith human samples, the antibody will be covered by the Abpromise. This means that if you arenot achieving thestaining expectedin yourtissue and you make usaware of this within 6 months ofpurchase you wouldbe entitled to afree replacementantibody or your money back.More information on the Abpromise canbe found from the followinglink:

    https://www.abcam.com/abpromise

    I hope this informationhas been of some help. If you requireany further information, please do not hesitate to contact us again.

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