Overview

  • Product name
  • Description
    Rabbit polyclonal to CXCR2
  • Host species
    Rabbit
  • Specificity
    Ab14935 detects CXCR2.
  • Tested applications
    Suitable for: IP, WB, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    EDLSNYSYSSTLPPFLLDAAPC

    , corresponding to amino acids 18-39 of Human CXCR2

  • General notes
    Concentration varies from batch to batch, usually 0.5 mg/ml to 0.7 mg/ml.

Properties

Applications

Our Abpromise guarantee covers the use of ab14935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/250.
WB 1/500. Detects a band of approximately 55 kDa (predicted molecular weight: 41 kDa).Can be blocked with CXCR2 peptide (ab209353).
IHC-Fr 1/200.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    Phosphorylated upon ligand binding; which is required for desensitization.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C chemokine receptor type 2 antibody
    • CD 182 antibody
    • CD182 antibody
    • CD182 antigen antibody
    • CDw128b antibody
    • Chemokine (CXC) receptor 2 antibody
    • CMKAR2 antibody
    • CXC-R2 antibody
    • CXCR 2 antibody
    • CXCR-2 antibody
    • CXCR2 antibody
    • CXCR2_HUMAN antibody
    • GRO/MGSA receptor antibody
    • High affinity interleukin-8 receptor B antibody
    • IL 8 receptor type 2 antibody
    • IL 8R B antibody
    • IL-8 receptor type 2 antibody
    • IL-8R B antibody
    • IL8 RB antibody
    • IL8 receptor type 2 antibody
    • IL8R B antibody
    • IL8R2 antibody
    • IL8RA antibody
    • Interleukin 8 Receptor B antibody
    • Interleukin 8 receptor, beta antibody
    • Interleukin 8 receptor, type 2 antibody
    see all

Images

  • WB of CXCR2-201AP and PC-CXCR2. Antibody dilution 1:500 in Diluobuffer

  • Anti-CXCR2 antibody (ab14935) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes
  • Immunohistochemical analysis of frozen mouse prostate tissue, staining CXCR2 with ab14935.

    Tissue was fixed in formaldehyde and blocked with 10% donkey serum and 1% BSA. Samples were incubated with primary antibody (1/100) and a FITC-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody.
  • ab14935 staining CXCR2 in Mouse pancreatic neoplasia associated stroma tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/250 in PBS) for 8 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab14935 staining CXCR2 in Mouse spleen cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab14935 staining CXCR2 in Human pancreatic neoplastic lesion tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. An Alexa Fluor®555-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Chen X  et al. Anti-Semaphorin-7A single chain antibody demonstrates beneficial effects on pulmonary inflammation during acute lung injury. Exp Ther Med 15:2356-2364 (2018). Read more (PubMed: 29456642) »
  • Sobel K  et al. Wnt-3a-activated human fibroblasts promote human keratinocyte proliferation and matrix destruction. Int J Cancer 136:2786-98 (2015). Read more (PubMed: 25403422) »
See all 14 Publications for this product

Customer reviews and Q&As

1-10 of 21 Abreviews or Q&A

Answer

Thank you for contacting us. although I cannot trace the resolution of that specific complaint from two years ago, it seems the Scientific Support Representative who published the review had contacted the reviewer regarding possible troubleshooting and our Abpromise. This means if no technical advice could resolve the problem, and it was within 6 months of purchase, the customer would receive a replacment, credit or refund for the product. This only applies to species and products we have tested in (for this antibody mouse, human, rat IP, WB, IHC-Fr, IHC-P, ICC/IF).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.
We currently only have available the one size listed on the datasheet for a particular antibody. We do not have any CXCR antibody panels at this time.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting Abcam regarding ab14935.


I have added a replacement of this antibody to Cedarlane's order.


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Answer

Thank you for confirming these details and for your cooperation.
Sorry again for the long time it took to resolve this problem.

The details provided enable us to closely monitor the quality of our products.

As requested, I have issued a free of charge replacement with ab61100.


Thank you for your help and cooperation. Please do not hesitate to contact us if you need anything further.

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Answer

Thank you for your reply.

An alternative anti-CXCR2 tested in Mouse and Western Blotis ab61100 (Rabbit polyclonal, https://www.abcam.com/ab61100). Please note that ab61100 is specific to the phosphorylated form of CXCR2, a milk blocking is therefore not recommended for Western Blot.

Would you agree to receive this antibody as a replacement?

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Answer

Sorry for the delay of my reply.
Unfortunately I have not been able to get more information about the expected molecular weight from the source of this anti-CXCR2 antibody ab14935.
I appreciate the time you have spent on these experiments as well as the time you had to wait for our reply.
In compensation Iwould be pleased to arrange a credit note againstthe original order or areplacement with an alternative anti-CXCR2 such as https://www.abcam.com/ab21641 or https://www.abcam.com/ab65968.


I look forward to hearing from you with details of how you would like to proceed.

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Question
Answer

Thank you for your call today and for keeping us updated about the results with ab24963.

As we discussed, I'm sending a free of charge vial of ab14935. This will arrive tomorrow on the order ***. Please keep me updated about how this antibody works for you, and if there is anything else that we can do for you, please let me know.

Have a great day!

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Answer



The source of the antibody ab14935 informed me that inwestern blot, positive controls onlyshow a 55 kDa diffuse band for CXCR2, however, in several tissues and cells lines the higher MW bands are seen and reported. This may be due to post-translational modifications on the protein and also some protein-protein interaction (trans-glutaminase cross linking) to give higher MW bands.

I have also been informed that ourcollaboratorswill see if there are other papers using this antibody showing this high MW band and have an explanation for that.

I will contact you again as soon as I receivesomeinformation. Thank you for your patience.

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Question
Answer

Thank you for your call today and for letting us know about the trouble with ab14935. As we discussed, I am sending a free of charge vial of ab65968 on the order ***, which should arrive tomorrow. Please keep me updated about the results using this replacement antibody, and let me know if there is anything else that we can do for you. Enjoy the rest of your week!

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Answer

Thank you for contacting us. The antibody ab14935 has been tested in COS1/Cos7 cells that do not express CXCR2, and it did not labelled the 55 kDa band. Furthermore, the antibody was also tested in cells that express CXCR2 and with recombinant CXCR2 protein. The cross reactivity with other chemokine receptors, has not yet been tested. As soon as we got further information, we will update the online datasheet accordingly. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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