Anti-CXCR2 antibody (ab217314)
Key features and details
- Rabbit polyclonal to CXCR2
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CXCR2 antibody
See all CXCR2 primary antibodies -
Description
Rabbit polyclonal to CXCR2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide within Human CXCR2 aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P25025 -
Positive control
- WB: mouse bone lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/1000. Predicted molecular weight: 40 kDa.
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Notes |
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WB
1/100 - 1/1000. Predicted molecular weight: 40 kDa. |
Target
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Function
Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsPhosphorylated upon ligand binding; which is required for desensitization. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12765 Mouse
- SwissProt: P35343 Mouse
- Unigene: 234466 Mouse
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Alternative names
- C-X-C chemokine receptor type 2 antibody
- CD 182 antibody
- CD182 antibody
see all
Images
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Anti-CXCR2 antibody (ab217314) at 1/300 dilution + mouse bone lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 40 kDaSample boiled at 95°C 10min before loading. We recommend blocking for 4 hours at room temperature or 2 hours at 37°C, rinsing more than five times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab217314 has been referenced in 7 publications.
- Yin X et al. Celecoxib alleviates zinc deficiency-promoted colon tumorigenesis through suppressing inflammation. Aging (Albany NY) 13:8320-8334 (2021). PubMed: 33686969
- Nam AR et al. ATR inhibition amplifies antitumor effects of olaparib in biliary tract cancer. Cancer Lett 516:38-47 (2021). PubMed: 34082024
- Xu M et al. Blockage of C-X-C Motif Chemokine Receptor 2 (CXCR2) Suppressed Uric Acid (UA)-Induced Cardiac Remodeling. Front Physiol 12:700338 (2021). PubMed: 34385934
- Roy S et al. Tumor Lymphatic Interactions Induce CXCR2-CXCL5 Axis and Alter Cellular Metabolism and Lymphangiogenic Pathways to Promote Cholangiocarcinoma. Cells 10:N/A (2021). PubMed: 34831316
- Wu D et al. Hypoxia-induced microRNA-141 regulates trophoblast apoptosis, invasion, and vascularization by blocking CXCL12ß/CXCR2/4 signal transduction. Biomed Pharmacother 116:108836 (2019). PubMed: 31004838
- Jin MH et al. Therapeutic Co-targeting of WEE1 and ATM Downregulates PD-L1 Expression in Pancreatic Cancer. Cancer Res Treat N/A:N/A (2019). PubMed: 31291716
- Nojiri T et al. Transcriptome analysis reveals a role for the endothelial ANP-GC-A signaling in interfering with pre-metastatic niche formation by solid cancers. Oncotarget 8:65534-65547 (2017). PubMed: 29029451