Anti-CXCR2 antibody (ab217314)
Key features and details
- Rabbit polyclonal to CXCR2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CXCR2 antibody
See all CXCR2 primary antibodies -
Description
Rabbit polyclonal to CXCR2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human CXCR2 aa 316-350 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:FIGQK FRHGLLKILA IHGLISKDSL PKDSRPSFVG
Database link: P25025 -
Positive control
- Rat hepatoma and human rectal carcinoma tissues; mouse bone lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab217314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/1000. Predicted molecular weight: 40 kDa. | |
| IHC-P | 1/100 - 1/500. |
Target
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Function
Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsPhosphorylated upon ligand binding; which is required for desensitization. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3579 Human
- Entrez Gene: 12765 Mouse
- Entrez Gene: 29385 Rat
- Omim: 146928 Human
- SwissProt: P25025 Human
- SwissProt: P35343 Mouse
- SwissProt: P35407 Rat
- Unigene: 846 Human
see all -
Alternative names
- C-X-C chemokine receptor type 2 antibody
- CD 182 antibody
- CD182 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody (ab217314)
Immunohistochemical analysis of formalin-fixed and paraffin embedded rat hepatoma tissue labeling CXCR2 using ab217314 at 1/100, followed by secondary detection and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody (ab217314)Immunohistochemical analysis of formalin-fixed and paraffin embedded human rectal carcinoma tissue labeling CXCR2 using ab217314 at 1/200, followed by secondary detection and DAB staining.
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Western blot - Anti-CXCR2 antibody (ab217314)Anti-CXCR2 antibody (ab217314) at 1/300 dilution + mouse bone lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 40 kDaSample boiled at 95°C 10min before loading. We recommend blocking for 4 hours at room temperature or 2 hours at 37°C, rinsing more than five times.
Protocols
Datasheets and documents
References (3)
ab217314 has been referenced in 3 publications.
- Wu D et al. Hypoxia-induced microRNA-141 regulates trophoblast apoptosis, invasion, and vascularization by blocking CXCL12ß/CXCR2/4 signal transduction. Biomed Pharmacother 116:108836 (2019). PubMed: 31004838
- Jin MH et al. Therapeutic Co-targeting of WEE1 and ATM Downregulates PD-L1 Expression in Pancreatic Cancer. Cancer Res Treat N/A:N/A (2019). PubMed: 31291716
- Nojiri T et al. Transcriptome analysis reveals a role for the endothelial ANP-GC-A signaling in interfering with pre-metastatic niche formation by solid cancers. Oncotarget 8:65534-65547 (2017). PubMed: 29029451
