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    cxcr2-antibody-ab217314.pdf

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Signal Transduction Signaling Pathway G Protein Signaling GPCR
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Anti-CXCR2 antibody (ab217314)

  • Datasheet
Submit a review Submit a question References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody (ab217314)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody (ab217314)
  • Western blot - Anti-CXCR2 antibody (ab217314)

Key features and details

  • Rabbit polyclonal to CXCR2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CXCR2 antibody
    See all CXCR2 primary antibodies
  • Description

    Rabbit polyclonal to CXCR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CXCR2 aa 316-350 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    FIGQK FRHGLLKILA IHGLISKDSL PKDSRPSFVG


    Database link: P25025
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Rat hepatoma and human rectal carcinoma tissues; mouse bone lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human CXCR2 protein (ab112309)

Applications

Our Abpromise guarantee covers the use of ab217314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function

    Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    Phosphorylated upon ligand binding; which is required for desensitization.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P25025 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3579 Human
    • Entrez Gene: 12765 Mouse
    • Entrez Gene: 29385 Rat
    • Omim: 146928 Human
    • SwissProt: P25025 Human
    • SwissProt: P35343 Mouse
    • SwissProt: P35407 Rat
    • Unigene: 846 Human
    • Unigene: 234466 Mouse
    • Unigene: 90347 Rat
    see all
  • Alternative names

    • C-X-C chemokine receptor type 2 antibody
    • CD 182 antibody
    • CD182 antibody
    • CD182 antigen antibody
    • CDw128b antibody
    • Chemokine (CXC) receptor 2 antibody
    • CMKAR2 antibody
    • CXC-R2 antibody
    • CXCR 2 antibody
    • CXCR-2 antibody
    • CXCR2 antibody
    • CXCR2_HUMAN antibody
    • GRO/MGSA receptor antibody
    • High affinity interleukin-8 receptor B antibody
    • IL 8 receptor type 2 antibody
    • IL 8R B antibody
    • IL-8 receptor type 2 antibody
    • IL-8R B antibody
    • IL8 RB antibody
    • IL8 receptor type 2 antibody
    • IL8R B antibody
    • IL8R2 antibody
    • IL8RA antibody
    • Interleukin 8 Receptor B antibody
    • Interleukin 8 receptor, beta antibody
    • Interleukin 8 receptor, type 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody (ab217314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody (ab217314)

    Immunohistochemical analysis of formalin-fixed and paraffin embedded rat hepatoma tissue labeling CXCR2 using ab217314 at 1/100, followed by secondary detection and DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody (ab217314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody (ab217314)

    Immunohistochemical analysis of formalin-fixed and paraffin embedded human rectal carcinoma tissue labeling CXCR2 using ab217314 at 1/200, followed by secondary detection and DAB staining.

  • Western blot - Anti-CXCR2 antibody (ab217314)
    Western blot - Anti-CXCR2 antibody (ab217314)
    Anti-CXCR2 antibody (ab217314) at 1/300 dilution + mouse bone lysate

    Secondary
    Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution

    Predicted band size: 40 kDa



    Sample boiled at 95°C 10min before loading. We recommend blocking for 4 hours at room temperature or 2 hours at 37°C, rinsing more than five times.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab217314? Please let us know so that we can cite the reference in this datasheet.

    ab217314 has been referenced in 3 publications.

    • Wu D  et al. Hypoxia-induced microRNA-141 regulates trophoblast apoptosis, invasion, and vascularization by blocking CXCL12ß/CXCR2/4 signal transduction. Biomed Pharmacother 116:108836 (2019). PubMed: 31004838
    • Jin MH  et al. Therapeutic Co-targeting of WEE1 and ATM Downregulates PD-L1 Expression in Pancreatic Cancer. Cancer Res Treat N/A:N/A (2019). PubMed: 31291716
    • Nojiri T  et al. Transcriptome analysis reveals a role for the endothelial ANP-GC-A signaling in interfering with pre-metastatic niche formation by solid cancers. Oncotarget 8:65534-65547 (2017). PubMed: 29029451

    Customer reviews and Q&As

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