Key features and details
- Rabbit polyclonal to CXCR2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CXCR2 antibody
See all CXCR2 primary antibodies
DescriptionRabbit polyclonal to CXCR2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- Rat hepatoma and human rectal carcinoma tissues; mouse bone lysate
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab217314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 40 kDa.|
|IHC-P||1/100 - 1/500.|
FunctionReceptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
modificationsPhosphorylated upon ligand binding; which is required for desensitization.
Cellular localizationCell membrane.
- Information by UniProt
- C-X-C chemokine receptor type 2 antibody
- CD 182 antibody
- CD182 antibody
Immunohistochemical analysis of formalin-fixed and paraffin embedded rat hepatoma tissue labeling CXCR2 using ab217314 at 1/100, followed by secondary detection and DAB staining.
Immunohistochemical analysis of formalin-fixed and paraffin embedded human rectal carcinoma tissue labeling CXCR2 using ab217314 at 1/200, followed by secondary detection and DAB staining.
Anti-CXCR2 antibody (ab217314) at 1/300 dilution + mouse bone lysate
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 40 kDa
Sample boiled at 95°C 10min before loading. We recommend blocking for 4 hours at room temperature or 2 hours at 37°C, rinsing more than five times.
ab217314 has been referenced in 3 publications.
- Wu D et al. Hypoxia-induced microRNA-141 regulates trophoblast apoptosis, invasion, and vascularization by blocking CXCL12ß/CXCR2/4 signal transduction. Biomed Pharmacother 116:108836 (2019). PubMed: 31004838
- Jin MH et al. Therapeutic Co-targeting of WEE1 and ATM Downregulates PD-L1 Expression in Pancreatic Cancer. Cancer Res Treat N/A:N/A (2019). PubMed: 31291716
- Nojiri T et al. Transcriptome analysis reveals a role for the endothelial ANP-GC-A signaling in interfering with pre-metastatic niche formation by solid cancers. Oncotarget 8:65534-65547 (2017). PubMed: 29029451