Anti-CXCR2 antibody (ab217314)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-CXCR2 antibody
See all CXCR2 primary antibodies -
Description
Rabbit polyclonal to CXCR2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human CXCR2 aa 316-350 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:FIGQK FRHGLLKILA IHGLISKDSL PKDSRPSFVG
Database link: P25025 -
Positive control
- Rat hepatoma and human rectal carcinoma tissues; mouse bone lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab217314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/1000. Predicted molecular weight: 40 kDa. | |
| IHC-P | 1/100 - 1/500. |
Target
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Function
Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsPhosphorylated upon ligand binding; which is required for desensitization. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3579 Human
- Entrez Gene: 12765 Mouse
- Entrez Gene: 29385 Rat
- Omim: 146928 Human
- SwissProt: P25025 Human
- SwissProt: P35343 Mouse
- SwissProt: P35407 Rat
- Unigene: 846 Human
see all -
Alternative names
- C-X-C chemokine receptor type 2 antibody
- CD 182 antibody
- CD182 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody (ab217314)Immunohistochemical analysis of formalin-fixed and paraffin embedded rat hepatoma tissue labeling CXCR2 using ab217314 at 1/100, followed by secondary detection and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR2 antibody (ab217314)Immunohistochemical analysis of formalin-fixed and paraffin embedded human rectal carcinoma tissue labeling CXCR2 using ab217314 at 1/200, followed by secondary detection and DAB staining.
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Western blot - Anti-CXCR2 antibody (ab217314)Anti-CXCR2 antibody (ab217314) at 1/300 dilution + mouse bone lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/500 dilution
Predicted band size: 40 kDa
Datasheets and documents
References
This product has been referenced in:
- Wu D et al. Hypoxia-induced microRNA-141 regulates trophoblast apoptosis, invasion, and vascularization by blocking CXCL12ß/CXCR2/4 signal transduction. Biomed Pharmacother 116:108836 (2019). Read more (PubMed: 31004838) »
- Nojiri T et al. Transcriptome analysis reveals a role for the endothelial ANP-GC-A signaling in interfering with pre-metastatic niche formation by solid cancers. Oncotarget 8:65534-65547 (2017). Read more (PubMed: 29029451) »
