Anti-CXCR2 antibody (ab65968)
Key features and details
- Rabbit polyclonal to CXCR2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-CXCR2 antibody
See all CXCR2 primary antibodies -
Description
Rabbit polyclonal to CXCR2 -
Host species
Rabbit -
Specificity
No cross reactivity with other proteins.
-
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human CXCR2 aa 196-212 (internal sequence). Different from the related mouse sequence by six amino acids.
Sequence:CYEDMGNNTANWRMLLR
-
Positive control
- WB: Rat spleen, rat kidney, rat brain, mouse testis, human rectal cancer, and mouse brain tissue lysates and HEPA whole cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
|
Notes |
---|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
Target
-
Function
Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsPhosphorylated upon ligand binding; which is required for desensitization. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3579 Human
- Entrez Gene: 12765 Mouse
- Entrez Gene: 29385 Rat
- Omim: 146928 Human
- SwissProt: P25025 Human
- SwissProt: P35343 Mouse
- SwissProt: P35407 Rat
- Unigene: 846 Human
see all -
Alternative names
- C-X-C chemokine receptor type 2 antibody
- CD 182 antibody
- CD182 antibody
see all
Images
-
All lanes : Anti-CXCR2 antibody (ab65968) at 0.5 µg/ml
Lane 1 : Rat spleen tissue lysate at 50 µg
Lane 2 : Rat kidney tissue lysate at 50 µg
Lane 3 : Rat brain tissue lysate at 50 µg
Lane 4 : Mouse testis tissue lysate at 50 µg
Lane 5 : HEPA whole cell lysate at 40 µg
Lane 6 : Mouse brain tissue lysate at 50 µg
Predicted band size: 41 kDa
Observed band size: 41 kDa -
All lanes : Anti-CXCR2 antibody (ab65968)
All lanes : Human rectal cancer tissue lysate
Predicted band size: 41 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (9)
ab65968 has been referenced in 9 publications.
- Liu F et al. Astragalus mongholicus Bunge and Curcuma aromatica Salisb. inhibits liver metastasis of colon cancer by regulating EMT via the CXCL8/CXCR2 axis and PI3K/AKT/mTOR signaling pathway. Chin Med 17:91 (2022). PubMed: 35922850
- Cai Q et al. Inflammation-Associated Senescence Promotes Helicobacter pylori-Induced Atrophic Gastritis. Cell Mol Gastroenterol Hepatol 11:857-880 (2021). PubMed: 33161156
- Zhang J et al. Determination of Potential Therapeutic Targets and Prognostic Markers of Ovarian Cancer by Bioinformatics Analysis. Biomed Res Int 2021:8883800 (2021). PubMed: 33829065
- Wang X et al. CXCL6 regulates cell permeability, proliferation, and apoptosis after ischemia-reperfusion injury by modulating Sirt3 expression via AKT/FOXO3a activation. Cancer Biol Ther 22:30-39 (2021). PubMed: 33241954
- Ando Y et al. Necroptosis in pancreatic cancer promotes cancer cell migration and invasion by release of CXCL5. PLoS One 15:e0228015 (2020). PubMed: 31999765
- Serdar M et al. Involvement of CXCL1/CXCR2 During Microglia Activation Following Inflammation-Sensitized Hypoxic-Ischemic Brain Injury in Neonatal Rats. Front Neurol 11:540878 (2020). PubMed: 33123073
- Schludi B et al. Islet inflammation and ductal proliferation may be linked to increased pancreatitis risk in type 2 diabetes. JCI Insight 2:N/A (2017). IHC-P, WB ; Rat, Human . PubMed: 28679961
- Lu B et al. Effect of CCL2 siRNA on proliferation and apoptosis in the U251 human glioma cell line. Mol Med Rep 16:3387-3394 (2017). PubMed: 28714025
- Kawamura M et al. CXCL5, a promoter of cell proliferation, migration and invasion, is a novel serum prognostic marker in patients with colorectal cancer. Eur J Cancer 48:2244-51 (2012). PubMed: 22197219