Overview

  • Product name

  • Description

    Rabbit polyclonal to CXCR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CXCR2 aa 196-212 (internal sequence).

  • Positive control

    • WB: Rat Spleen, Rat Kidney, Rat Brain, Mouse Testis, Human Rectal Cancer, HEPA and Mouse Brain lysate. IHC-P: human breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab65968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    Phosphorylated upon ligand binding; which is required for desensitization.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C-X-C chemokine receptor type 2 antibody
    • CD 182 antibody
    • CD182 antibody
    • CD182 antigen antibody
    • CDw128b antibody
    • Chemokine (CXC) receptor 2 antibody
    • CMKAR2 antibody
    • CXC-R2 antibody
    • CXCR 2 antibody
    • CXCR-2 antibody
    • CXCR2 antibody
    • CXCR2_HUMAN antibody
    • GRO/MGSA receptor antibody
    • High affinity interleukin-8 receptor B antibody
    • IL 8 receptor type 2 antibody
    • IL 8R B antibody
    • IL-8 receptor type 2 antibody
    • IL-8R B antibody
    • IL8 RB antibody
    • IL8 receptor type 2 antibody
    • IL8R B antibody
    • IL8R2 antibody
    • IL8RA antibody
    • Interleukin 8 Receptor B antibody
    • Interleukin 8 receptor, beta antibody
    • Interleukin 8 receptor, type 2 antibody
    see all

Images

  • All lanes : Anti-CXCR2 antibody (ab65968) at 0.5 µg/ml

    Lane 1 : Rat spleen tissue at 50 µg
    Lane 2 : Rat kidney tissue at 50 µg
    Lane 3 : Rat brain tissue at 50 µg
    Lane 4 : Mouse testis tissue at 50 µg
    Lane 5 : HEPA Whole Cell Lysate at 40 µg
    Lane 6 : Mouse brain tissue lysate at 50 µg

    Predicted band size: 41 kDa

  • Anti-CXCR2 antibody (ab65968) at 2 µg/ml + Human colon carcinoma lysate

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

  • IHC image of ab65968 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65968, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Schludi B  et al. Islet inflammation and ductal proliferation may be linked to increased pancreatitis risk in type 2 diabetes. JCI Insight 2:N/A (2017). IHC-P, WB ; Rat, Human . Read more (PubMed: 28679961) »
  • Lu B  et al. Effect of CCL2 siRNA on proliferation and apoptosis in the U251 human glioma cell line. Mol Med Rep 16:3387-3394 (2017). Read more (PubMed: 28714025) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

Thank you for your call today and for letting us know about the trouble with ab14935. As we discussed, I am sending a free of charge vial of ab65968 on the order ***, which should arrive tomorrow. Please keep me updated about the results using this replacement antibody, and let me know if there is anything else that we can do for you. Enjoy the rest of your week!

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (human colon tumor cell-HCT116)
Loading amount
40 µg
Specification
human colon tumor cell-HCT116
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 16 2011

Answer

Thank you for submitting an Abreview for ab25968. We greatly appreciate your feedback and strive to publish both negative and positive reviews, rewarding each with Abpoints. Before we publish negative Abreviews our Scientific Support team reviews each one. I am wondering whether the faint band is in fact the CXCR2 band or the stronger band a bit higher. Often bands will appear a few kD off from the projected size based on modifications, or even gel and buffer type. You may wish to try another cell type that is CXCR2 decifient for comparison. We do guarantee all our products for the tested species and application within 6 months of purchase. If you are unhappy with your results within this time frame please send us your order number and we can offer a free of charge replacement, credit or refund. Thank you very much for your time and we look forward to your reply.

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