Overview

  • Product name

    Anti-CXCR2 antibody [EPR22301-103]
    See all CXCR2 primary antibodies
  • Description

    Rabbit monoclonal [EPR22301-103] to CXCR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IP or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CXCR2 aa 1-100. The exact sequence is proprietary.
    Database link: P25025

  • Positive control

    • IHC-P: Human spleen, gastric adenocarcinoma and pancreas adenocarcinoma tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IP or WB.
  • Target

    • Function

      Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Post-translational
      modifications

      Phosphorylated upon ligand binding; which is required for desensitization.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • C-X-C chemokine receptor type 2 antibody
      • CD 182 antibody
      • CD182 antibody
      • CD182 antigen antibody
      • CDw128b antibody
      • Chemokine (CXC) receptor 2 antibody
      • CMKAR2 antibody
      • CXC-R2 antibody
      • CXCR 2 antibody
      • CXCR-2 antibody
      • CXCR2 antibody
      • CXCR2_HUMAN antibody
      • GRO/MGSA receptor antibody
      • High affinity interleukin-8 receptor B antibody
      • IL 8 receptor type 2 antibody
      • IL 8R B antibody
      • IL-8 receptor type 2 antibody
      • IL-8R B antibody
      • IL8 RB antibody
      • IL8 receptor type 2 antibody
      • IL8R B antibody
      • IL8R2 antibody
      • IL8RA antibody
      • Interleukin 8 Receptor B antibody
      • Interleukin 8 receptor, beta antibody
      • Interleukin 8 receptor, type 2 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CXCR2 using ab225732 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Positive staining in neutrophils of human spleen (PMID: 29661142) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody.

       

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling CXCR2 using ab225732 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Positive staining in stromal cells of human gastric adenocarcinoma (PMID: 28575019) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of pancreas tissue labeling CXCR2 using ab225732 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Positive staining inn stromal cells of human adenocarcinoma of pancreas (PMID:27265504) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling CXCR2 using ab225732 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Negative control: No staining on human cerebrum (PMID: 28529637) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    References

    ab225732 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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