Overview

  • Product name

    Anti-CXCR2 antibody [MM0222-7J40]
    See all CXCR2 primary antibodies
  • Description

    Mouse monoclonal [MM0222-7J40] to CXCR2
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, Neutralising, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Human recombinant CXCR2

Properties

Applications

Our Abpromise guarantee covers the use of ab89254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/20 - 1/100.
Neutralising Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/200.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    Phosphorylated upon ligand binding; which is required for desensitization.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C-X-C chemokine receptor type 2 antibody
    • CD 182 antibody
    • CD182 antibody
    • CD182 antigen antibody
    • CDw128b antibody
    • Chemokine (CXC) receptor 2 antibody
    • CMKAR2 antibody
    • CXC-R2 antibody
    • CXCR 2 antibody
    • CXCR-2 antibody
    • CXCR2 antibody
    • CXCR2_HUMAN antibody
    • GRO/MGSA receptor antibody
    • High affinity interleukin-8 receptor B antibody
    • IL 8 receptor type 2 antibody
    • IL 8R B antibody
    • IL-8 receptor type 2 antibody
    • IL-8R B antibody
    • IL8 RB antibody
    • IL8 receptor type 2 antibody
    • IL8R B antibody
    • IL8R2 antibody
    • IL8RA antibody
    • Interleukin 8 Receptor B antibody
    • Interleukin 8 receptor, beta antibody
    • Interleukin 8 receptor, type 2 antibody
    see all

References

ab89254 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for your recent telephone enquiry.

I am pleased to have received some furhter ifnormation from the originator for you. They have confirmed that no special IHC-Fr procedure woudl be required, you can use a standard procedure such as the one they have provided, which I have copied below. However, as with any general protocol please note this is a guideline only and may require someindividual optimization.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

General Protocol for IHC on Frozen sections

Cryopreservation of Tissues:

1. Immediately snap freeze fresh tissue in isopentane mixed with dry ice, and keep at -70 °C. Do not allow frozen tissue to thaw before cutting.
2. Embed the tissue completely in OCT compound prior to cryostat sectioning.

Sectioning:

3. Cut cryostat sections at 5-10 ìm and mount on gelatin-coated histological slides.
4. Air dry the sections for 30 minutes at room temperature to prevent sections from
falling off the slides during antibody incubations (Note: Slides can be stored
unfixed at -80 oC).

Fixing:
5. Immediately put slides into a slides jar containing pre-cold (-20°C) Fixation
Buffer (i.e. acetone, or methanol et al) upon removal from the freezer.
6. Fix the sections at -20°C for 20 minutes.
Staining:
7. Rehydrate the slides in wash buffer (PBS) for 10 minutes. Drain the excess wash
buffer.
8. Surround the tissue with a hydrophobic barrier using a barrier pen.
9. To quench endogenous peroxidase activity, incubate the sample with 1-3 drops
peroxidase blocking reagent (3% H2O2 in water or methanol) for 5-15 minutes.
10. Rinse the sample, and gently wash in wash buffer for 5 minutes.
11. To reduce non-specific hydrophobic interactions between the primary antibodies
and the tissue, block the section with 1-3 drops of serum blocking reagent for 15
minutes. Drain the slides, and wipe away any excess blocking reagent before
proceeding to the next step. Do not rinse.
12. Incubate the sample with primary antibodies (either rat anti-mouse or mouse antihuman)
in Incubation Buffer (1% BSA, 5% normal donkey serum, 0.3% Triton
X-100, and 0.01% sodium azide in PBS) overnight at 4°C (Following
manufacturer’s recommendations regarding working dilution for the primary
antibody).
13. Rinse the sample with wash buffer. Wash 3 times with wash buffer for 5 minutes
each, and drain the slides.
14. Incubate the sample with 1-3 polymer HRP conjugated anti-rat or anti mousesecondary
antibodies for 30-60 minutes.
15. Rinse with wash buffer 3 times for 5 minutes each and drain the slides.
Calculate the working volume of DAB Chromogen Solution given that 100-200
ìL is required to cover the entire tissue section on a slide. Add 1-5 drops of
DAB/AEC Chromogen Solution to cover the entire tissue section, and incubate
for 3-20 minutes. Monitor intensity of the tissue staining under a microscope.
Colored precipitate will localize to the sites of antigen expression as the
chromogenic substrate is converted by HRP enzyme into insoluble end product.
17. Rinse the sample with wash buffer 3 times for 10 minutes each.
18. Rinse in deionized H2O and drain the slides.
19. Stained tissue can be mounted either without nuclear counterstaining or
counterstained with nuclear counterstain hematoxylin for better visualization of
the tissue morphology.
20. Cover stained tissue with a coverslip of an appropriate size, place slides vertically
on a filter paper or towel to drain excess mounting medium and allow them to dry.

Read More

Answer

Thank you for contacting us.

With antibodies these two terms have similar meaning i.e. antibodies inhibit or neutralizes the cellular response either by binding to receptors, ligand or other cell signalling downstream proteins.

ab10401, ab89251, ab89254 are the antibodies against the receptors so you can use anyofthese antibodies without any hesitation.

Few years back we used the term inhibition to describe the inhibitor or neutralizing affect of antibody however recently we are using neutralizing because this applications covers wide range of our product list. ab10401 is one of our oldest product in catalogue.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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