Overview

  • Product name
    Anti-CXCR3 antibody [49801]
    See all CXCR3 primary antibodies
  • Description
    Mouse monoclonal [49801] to CXCR3
  • Host species
    Mouse
  • Specificity
    The clone number has been updated from (2Ar1) to (49801) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, Neutralising, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    NS0 cells transfected with human CXCR3.

  • Positive control
    • ICC/IF: HUVEC cells. IHC-P: Pig uterus tissue; human glioblastoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab64714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Neutralising Use a concentration of 1 - 6 µg/ml. in the presence of 7ng/ml of rhl-TAC, using the hCXCR3 transfected BaF/3 cells in a chemotaxis assay.
Flow Cyt Use a concentration of 5 - 10 µg/ml.

Add 10µl to 1-2.5x105 cells in total reaction volume 200µl.

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
    Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
    Isoform 3 mediates the activity of CXCL11.
  • Tissue specificity
    Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-X-C chemokine receptor type 3 antibody
    • CD 183 antibody
    • CD182 antibody
    • CD183 antibody
    • Chemokine (C X C motif) receptor 3 antibody
    • Chemokine (C X C) receptor 3 antibody
    • Chemokine CXC Motif Receptor 3 antibody
    • CKR L2 antibody
    • CKR-L2 antibody
    • CKRL2 antibody
    • CMKAR3 antibody
    • CXC-R3 antibody
    • CXCR-3 antibody
    • CXCR3 antibody
    • CXCR3_HUMAN antibody
    • G Protein Coupled Receptor 9 antibody
    • G protein-coupled receptor 9 antibody
    • GPR9 antibody
    • Interferon-inducible protein 10 receptor antibody
    • IP-10 receptor antibody
    • IP10 antibody
    • IP10 R antibody
    • IP10 receptor antibody
    • IP10-R antibody
    • IP10R antibody
    • Mig R antibody
    • Mig receptor antibody
    • Mig-R antibody
    • MIGR antibody
    see all

Images

  • ab64714 staining CXCR3 in primary cultured HUVEC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/400 in PBS + 2.5% serum) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab64714 staining CXCR3 in pig uterus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to enzymatic antigen retrieval prior to blocking with 10% serum for 20 minutes at 24°C. The primary antibody was diluted 1/200 and incubated with the sample for 30 minutes at 24°C. A biotin-conjugated ABC kit was used to detect the antibody.

    See Abreview

  • The chemoattractive activity of recombinant human CXCL11 (7 ng/ml) towards B cells transfected with human CXCR3 is neutralized in a dose-dependent manner by ab64714 (green). The IC50 for neutralization is typically 0.3 - 1.5 μg/ml.

  • Flow Cytometry of HEK293 cells transfected with human CXCR3 (upper histogram) or with irrelevant transfectants and eGFP (lower histogram) were stained with ab64714(10μl undiluted antibody per 1 x10cells) followed by APC conjugated anti-mouse IgG secondary antibody.

  • ab64714 staining CXCR3 in Human glioblastoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer prior to blocking with a proprietary non-protein reagent for 5 minutes at 25°C. The primary antibody was diluted 1/2000 and incubated with the sample for 30 minutes. A biotin-conjugated goat anti-mouse IgG was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Urra S  et al. Differential expression profile of CXCR3 splicing variants is associated with thyroid neoplasia. Potential role in papillary thyroid carcinoma oncogenesis? Oncotarget 9:2445-2467 (2018). Read more (PubMed: 29416784) »
  • Fang J  et al. The Expression of CXCL10/CXCR3 and Effect of the Axis on the Function of T Lymphocyte Involved in Oral Lichen Planus. Inflammation N/A:N/A (2018). Read more (PubMed: 30467622) »
See all 13 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ER1
Permeabilization
No
Specification
Tonsil
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 03 2016

Application
Flow Cytometry
Sample
Cow Cell (lymphocytes)
Permeabilization
No
Gating Strategy
lymphocytes
Specification
lymphocytes
Preparation
Cell harvesting/tissue preparation method: Ficoll gradient
Sample buffer: Optimized PBS (BD Cellwash)
Fixation
none

Abcam user community

Verified customer

Submitted Jul 21 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (spleen, lymphe nodes)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0 / Tris-EDTA buffer pH 9.0 / Proteinase K
Permeabilization
No
Specification
spleen, lymphe nodes
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 21 2015

Answer

Thank you for contacting us.

Both antibodies use PBST or TBST at pH 7.4-7.6 in 5% milk.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your inquiry.

The immunogen for this antibody was the full length human CXCR3 (aa1-368, according to Accession # P49682).

To our knowledge the exact epitope was not further mapped.

I am sorry that I could not provide you with a more detailed answer and hope this information is nevertheless helpful.

Read More

Answer

Gracias por tu respuesta.
Si os interesa testar el anticuerpo en ratón, lo primero que tenéis que hacer es indicármelo para que yo os envíe el código canjeable por un anticuerpo gratuito en la siguiente compra.
Una vez te mande el código, podéis hacer el pedido como lo hacéis normalmente, es decir, a través de nuestro distribuidor autorizado en Argentina (Tecnolab).
Cuando os llegue el anticuerpo lo probáis en ratón, y nos mandáis los resultados bajo forma de Abreview:
https://www.abcam.com/index.html?pageconfig=resource&rid=10332&viapagetrap=abreviews
En el momento en que recibamos los resultados, el código que os he enviado se activa, y puede utilizarse para el siguiente pedido, que os saldrá completamente gratuito. El código tiene una validez de 4 meses.
Espero haber resuelto tus dudas, si tienes alguna otra consulta, no dudes en volvernos a contactar.

Read More

Answer

Thank you for contacting us.
Unfortunately we have not tested this antibody in mouse samples so do not have any experimental evidence of the use of it with this species.
The epitope is not mapped, so it is not possible to predict the extent of reactivity of this antibody with mouse tissue samples.
I am sorry I don’t have more information available. In any case, if you are interested in participating in our antibodies evaluation system (Abreview), and test ab64714 with Mouse I will be happy to offer a discount code for a future purchase if you submit the results (in a form of an Abreview) to our website.
It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.
If you are interested in this offer, please let me know before placing the order.
If you want to read more about the Abreview system, please have a look at the next link:
https://www.abcam.com/index.html?pageconfig=resource&rid=10332&viapagetrap=abreviews
Please let me know if you would be interested in this program, and if you have any other doubts about it. I am happy to help, and clarify it further if necessary.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Glioblastoma)
Specification
Glioblastoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
proprietary non-protein reagent as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C

Dr. R Berahovich

Verified customer

Submitted Nov 30 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Uterus)
Specification
Uterus
Fixative
Paraformaldehyde
Antigen retrieval step
Enzymatic
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C

Frank White

Verified customer

Submitted Oct 20 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Primary cultured HUVECs)
Specification
Primary cultured HUVECs
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Bing Wang

Verified customer

Submitted Aug 26 2010

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