Anti-CXCR3 antibody [49801] (ab64714)
- Datasheet
- References (13)
- Protocols
Overview
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Product nameAnti-CXCR3 antibody [49801]
See all CXCR3 primary antibodies -
DescriptionMouse monoclonal [49801] to CXCR3
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Host speciesMouse
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SpecificityThe clone number has been updated from (2Ar1) to (49801) both clone numbers name the same antibody clone.
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Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, Neutralising, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
NS0 cells transfected with human CXCR3.
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Positive control
- ICC/IF: HUVEC cells. IHC-P: Pig uterus tissue; human glioblastoma tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 40% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: None
Constituents: 5% Trehalose, PBS, pH 7.2 -
Concentration information loading... -
PurityAscites
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Purification notesPurified from ascites.
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ClonalityMonoclonal
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Clone number49801
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MyelomaNS0
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IsotypeIgG1
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Research areas
Associated products
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Alternative Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab64714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| Neutralising | Use a concentration of 1 - 6 µg/ml. in the presence of 7ng/ml of rhl-TAC, using the hCXCR3 transfected BaF/3 cells in a chemotaxis assay. | |
| Flow Cyt | Use a concentration of 5 - 10 µg/ml. Add 10µl to 1-2.5x105 cells in total reaction volume 200µl.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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FunctionReceptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
Isoform 3 mediates the activity of CXCL11. -
Tissue specificityIsoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 2833 Human
- Omim: 300574 Human
- SwissProt: P49682 Human
- Unigene: 198252 Human
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Alternative names
- C-X-C chemokine receptor type 3 antibody
- CD 183 antibody
- CD182 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-CXCR3 antibody [49801] (ab64714)](https://www.abcam.com/ps/products/64/ab64714/Images/CXCR3-Primary-antibodies-ab64714-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CXCR3 antibody [49801] (ab64714)This image is courtesy of an Abreview submitted by Bing Wangab64714 staining CXCR3 in primary cultured HUVEC by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 5% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/400 in PBS + 2.5% serum) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR3 antibody [49801] (ab64714)](https://www.abcam.com/ps/products/64/ab64714/Images/CXCR3-Primary-antibodies-ab64714-8.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR3 antibody [49801] (ab64714)This image is courtesy of an Abreview submitted by Frank Whiteab64714 staining CXCR3 in pig uterus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to enzymatic antigen retrieval prior to blocking with 10% serum for 20 minutes at 24°C. The primary antibody was diluted 1/200 and incubated with the sample for 30 minutes at 24°C. A biotin-conjugated ABC kit was used to detect the antibody. -
![Neutralising - Anti-CXCR3 antibody [49801] (ab64714)](https://www.abcam.com/ps/products/64/ab64714/Images/ab64714-308485-714.jpg)
Neutralising - Anti-CXCR3 antibody [49801] (ab64714)The chemoattractive activity of recombinant human CXCL11 (7 ng/ml) towards B cells transfected with human CXCR3 is neutralized in a dose-dependent manner by ab64714 (green). The IC50 for neutralization is typically 0.3 - 1.5 μg/ml.
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![Flow Cytometry - Anti-CXCR3 antibody [49801] (ab64714)](https://www.abcam.com/ps/products/64/ab64714/Images/ab64714-310712-FC-image.jpg)
Flow Cytometry - Anti-CXCR3 antibody [49801] (ab64714)Flow Cytometry of HEK293 cells transfected with human CXCR3 (upper histogram) or with irrelevant transfectants and eGFP (lower histogram) were stained with ab64714(10μl undiluted antibody per 1 x106 cells) followed by APC conjugated anti-mouse IgG secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR3 antibody [49801] (ab64714)](https://www.abcam.com/ps/products/64/ab64714/Images/CXCR3-Primary-antibodies-ab64714-15.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR3 antibody [49801] (ab64714)This image is courtesy of an Abreview submitted by R Berahovich, ChemoCentryx Inc, United Statesab64714 staining CXCR3 in Human glioblastoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate buffer prior to blocking with a proprietary non-protein reagent for 5 minutes at 25°C. The primary antibody was diluted 1/2000 and incubated with the sample for 30 minutes. A biotin-conjugated goat anti-mouse IgG was used as the secondary antibody.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Urra S et al. Differential expression profile of CXCR3 splicing variants is associated with thyroid neoplasia. Potential role in papillary thyroid carcinoma oncogenesis? Oncotarget 9:2445-2467 (2018). Read more (PubMed: 29416784) »
- Fang J et al. The Expression of CXCL10/CXCR3 and Effect of the Axis on the Function of T Lymphocyte Involved in Oral Lichen Planus. Inflammation N/A:N/A (2018). Read more (PubMed: 30467622) »
