Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CXCR3 antibody [2Ar1]

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (spleen, lymphe nodes)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0 / Tris-EDTA buffer pH 9.0 / Proteinase K
Permeabilization
No
Specification
spleen, lymphe nodes
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween 0.05%
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: LSAB-Kit DAKO K5003
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

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Submitted Jul 21 2015

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