Key features and details
- Rabbit polyclonal to CXCR3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CXCR3 antibody
See all CXCR3 primary antibodies
DescriptionRabbit polyclonal to CXCR3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
- This antibody gave a positive signal in the following human tissue lysates: Colon, Small Intestine, Lymph Node and Heart IF/ICC: HeK293 cell line
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab71864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 41 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionReceptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
Isoform 3 mediates the activity of CXCL11.
Tissue specificityIsoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- C-X-C chemokine receptor type 3 antibody
- CD 183 antibody
- CD182 antibody
ICC/IF image of ab71864 stained HEK293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-CXCR3 antibody (ab71864) at 1 µg/ml
Lane 1 : Human colon tissue lysate - total protein (ab30051)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Our observed 70kDa band is supported by the following publications: http://www.jbc.org/content/279/29/30219.long and http://www.jimmunol.org/cgi/content/full/173/10/6234
ab71864 has been referenced in 2 publications.
- Guan C et al. Antroquinonol Exerts Immunosuppressive Effect on CD8+ T Cell Proliferation and Activation to Resist Depigmentation Induced by H2O2. Oxid Med Cell Longev 2017:9303054 (2017). PubMed: 29456788
- O'Beirne SL et al. CXCL9 Regulates TGF-ß1-Induced Epithelial to Mesenchymal Transition in Human Alveolar Epithelial Cells. J Immunol 195:2788-96 (2015). PubMed: 26268659